Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to CYR61 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse, Human
Product nameAnti-CYR61 antibody - BSA and Azide free (Capture)
See all CYR61 primary antibodies
DescriptionRabbit monoclonal to CYR61 - BSA and Azide free (Capture)
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Mouse, Human
ab244801 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse CYR61. The recommended pair for sandwich ELISA is:
Capture: ab244801, Human/Mouse CYR61 Capture Antibody (unconjugated)
Detector: ab245074, Human/Mouse CYR61 Detector Antibody (unconjugated)
The reference range value is 31.3 - 3000 pg/ml for human and 62.5 - 4000 pg/ml for mouse.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab244801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal to CYR61 - BSA and Azide free (Detector) (ab245074).|
RelevanceCYR61 is a secreted, cysteine rich, heparin binding protein encoded by a growth factor inducible immediate early gene. It promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion, and appears to play a role in wound healing by up regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA A, VEGA C, MMP1, MMP3, TIMP1, uPA, PAI 1 and integrins alpha 3 and alpha 5. Cyr61 mediated gene regulation is dependent on heparin binding. CYR61 down regulates the expression of alpha 1 and alpha 2 subunits of collagen type 1. It promotes cell adhesion and adhesive signaling through integrin alpha 6/beta 1, cell migration through integrin alpha v/beta 5 and cell proliferation through integrin alpha v/beta 3.
- CCN1 antibody
- CYR61 protein precursor antibody
- CYR61 protein precursor antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244801 has not yet been referenced specifically in any publications.