Product nameAnti-CYR61/CCN1 antibody
See all CYR61/CCN1 primary antibodies
DescriptionRabbit polyclonal to CYR61/CCN1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human CYR61/CCN1 aa 350-450 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Hela nuclear cell extract Hela cell lysate. A431 cell lysate. Jurkat cell lysate Liver (Mouse) tissue lysate Heart (Mouse) tissue lysate Kidney (Mouse) tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab10760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42-50 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
ab10760 staining CYR61/CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary.
All lanes : Anti-CYR61/CCN1 antibody (ab10760) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 35 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-CYR61/CCN1 antibody (ab10760) at 1/500 dilution
Lane 1 : Hela nuclear cell extract
Lane 2 : Hela whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Western blot for CYR61/CCN1 antibody (ab10760) at 1/500 tested on the following human tissue lysates:
Lane 1: hela nuclear
Lane 2: hela
Lane 3: A431
Lane 4: Jurkat
Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700) [1/5000]
Expected molecular weight: 42 kDa Observed molecular weight: ~50 kDa
20µg cell lysate loaded per lane.