Key features and details
- Rabbit polyclonal to CYR61/CCN1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CYR61/CCN1 antibody
See all CYR61/CCN1 primary antibodies
DescriptionRabbit polyclonal to CYR61/CCN1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human CYR61/CCN1 aa 350-450 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Hela nuclear cell extract Hela cell lysate. A431 cell lysate. Jurkat cell lysate Liver (Mouse) tissue lysate Heart (Mouse) tissue lysate Kidney (Mouse) tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab10760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42-50 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
All lanes : Anti-CYR61/CCN1 antibody (ab10760) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 35 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-CYR61/CCN1 antibody (ab10760) at 1/500 dilution
Lane 1 : Hela nuclear cell extract
Lane 2 : Hela whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Western blot for CYR61/CCN1 antibody (ab10760) at 1/500 tested on the following human tissue lysates:
Lane 1: hela nuclear
Lane 2: hela
Lane 3: A431
Lane 4: Jurkat
Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700) [1/5000]
Expected molecular weight: 42 kDa Observed molecular weight: ~50 kDa
20µg cell lysate loaded per lane.
ab10760 has been referenced in 2 publications.
- Xiang T et al. Vasculogenic mimicry formation in EBV-associated epithelial malignancies. Nat Commun 9:5009 (2018). PubMed: 30479336
- Ikegame M et al. Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation. Biomed Res 37:117-26 (2016). PubMed: 27108881