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Synthetic peptide corresponding to Human CYR61/CCN1 aa 350-450 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
Our Abpromise guarantee covers the use of ab10760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab10760 staining CYR61/CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary.
Western blot for CYR61/CCN1 antibody (ab10760) at 1/500 tested on the following human tissue lysates:
Lane 1: hela nuclear
Lane 2: hela
Lane 3: A431
Lane 4: Jurkat
Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700) [1/5000]
Expected molecular weight: 42 kDa Observed molecular weight: ~50 kDa
20µg cell lysate loaded per lane.
ab10760 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"