Product nameAnti-CYR61/CCN1 antibody
See all CYR61/CCN1 primary antibodies
DescriptionRabbit polyclonal to CYR61/CCN1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Chimpanzee
Recombinant fragment within Human CYR61/CCN1 (internal sequence). The exact sequence is proprietary.
Database link: O00622
- WB: Brefeldin A-treated HeLa whole cell extract. IHC-P: Rat intestine tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
All lanes : Anti-CYR61/CCN1 antibody (ab228592) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 1X Brefeldin A for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS-PAGE gel.
Paraffin-embedded rat intestine tissue stained for CYR61/CCN1 using ab228592 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 5 µg/mL Brefeldin A for 24 hours (right panel), stained for CYR61/CCN1 (green) using ab228592 at 1/200 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
ab228592 has not yet been referenced specifically in any publications.