Overview

  • Product name

  • Description

    Rabbit polyclonal to CYR61/CCN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment within Human CYR61/CCN1 (internal sequence). The exact sequence is proprietary.
    Database link: O00622

  • Positive control

    • WB: Brefeldin A-treated HeLa whole cell extract. IHC-P: Rat intestine tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3CH61 antibody
    • CCN 1 antibody
    • CCN family member 1 antibody
    • CCN1 antibody
    • CYR 61 antibody
    • Cyr61 antibody
    • CYR61 protein antibody
    • CYR61_HUMAN antibody
    • Cysteine rich angiogenic inducer 61 antibody
    • Cysteine rich heparin binding protein 61 antibody
    • Cysteine-rich angiogenic inducer 61 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • IBP-10 antibody
    • IGF-binding protein 10 antibody
    • Igfbp 10 antibody
    • IGFBP-10 antibody
    • Igfbp10 antibody
    • Insulin like growth factor binding protein 10 antibody
    • Insulin-like growth factor-binding protein 10 antibody
    • Protein CYR61 antibody
    • Protein GIG1 antibody
    see all

Images

  • All lanes : Anti-CYR61/CCN1 antibody (ab228592) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 1X Brefeldin A for 24 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded rat intestine tissue stained for CYR61/CCN1 using ab228592 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 5 µg/mL Brefeldin A for 24 hours (right panel), stained for CYR61/CCN1 (green) using ab228592 at 1/200 dilution in ICC/IF. 

    Nuclear counter stain: Hoechst 33342 (blue).

References

ab228592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228592.
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