Anti-CYR61/CCN1 antibody (ab228592)
Key features and details
- Rabbit polyclonal to CYR61/CCN1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CYR61/CCN1 antibody
See all CYR61/CCN1 primary antibodies -
Description
Rabbit polyclonal to CYR61/CCN1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Chimpanzee -
Immunogen
Recombinant fragment within Human CYR61/CCN1 (internal sequence). The exact sequence is proprietary.
Database link: O00622 -
Positive control
- WB: Brefeldin A-treated HeLa whole cell extract. IHC-P: Rat intestine tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 42 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 42 kDa. |
Target
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Function
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 508941 Cow
- Entrez Gene: 3491 Human
- Entrez Gene: 16007 Mouse
- Entrez Gene: 83476 Rat
- Omim: 602369 Human
- SwissProt: A5A6L1 Chimpanzee
- SwissProt: O00622 Human
- SwissProt: P18406 Mouse
see all -
Alternative names
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
see all
Images
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All lanes : Anti-CYR61/CCN1 antibody (ab228592) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 1X Brefeldin A for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Paraffin-embedded rat intestine tissue stained for CYR61/CCN1 using ab228592 at 1/500 dilution in immunohistochemical analysis.
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 5 µg/mL Brefeldin A for 24 hours (right panel), stained for CYR61/CCN1 (green) using ab228592 at 1/200 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab228592 has been referenced in 5 publications.
- Gan YR et al. Dickkopf-1/cysteine-rich angiogenic inducer 61 axis mediates palmitic acid-induced inflammation and apoptosis of vascular endothelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33300071
- Zhang Y et al. Inhibin βA is an independent prognostic factor that promotes invasion via Hippo signaling in non‑small cell lung cancer. Mol Med Rep 24:N/A (2021). PubMed: 34505633
- Li W et al. Diagnostic value of using exosome-derived cysteine-rich protein 61 as biomarkers for acute coronary syndrome. Exp Ther Med 22:1437 (2021). PubMed: 34721679
- Liang X et al. Comparative Proteomic Analysis of tPVAT during Ang II Infusion. Biomedicines 9:N/A (2021). PubMed: 34944635
- Zhang J et al. K-RasG12V/Y40C-PI3K/AKT pathway regulates H1.4S35ph through PKA to promote the occurrence and development of osteosarcoma cancer. Artif Cells Nanomed Biotechnol 47:2048-2057 (2019). PubMed: 31126199