Key features and details
- Rabbit polyclonal to CYR61/CCN1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-CYR61/CCN1 antibody
See all CYR61/CCN1 primary antibodies
DescriptionRabbit polyclonal to CYR61/CCN1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Chimpanzee
Recombinant fragment within Human CYR61/CCN1 (internal sequence). The exact sequence is proprietary.
Database link: O00622
- WB: Brefeldin A-treated HeLa whole cell extract. IHC-P: Rat intestine tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- 3CH61 antibody
- CCN 1 antibody
- CCN family member 1 antibody
All lanes : Anti-CYR61/CCN1 antibody (ab228592) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 1X Brefeldin A for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS-PAGE gel.
Paraffin-embedded rat intestine tissue stained for CYR61/CCN1 using ab228592 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 5 µg/mL Brefeldin A for 24 hours (right panel), stained for CYR61/CCN1 (green) using ab228592 at 1/200 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228592 has been referenced in 1 publication.
- Zhang J et al. K-RasG12V/Y40C-PI3K/AKT pathway regulates H1.4S35ph through PKA to promote the occurrence and development of osteosarcoma cancer. Artif Cells Nanomed Biotechnol 47:2048-2057 (2019). PubMed: 31126199