Recombinant
RabMAb

Recombinant Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

Overview

  • Product name

    Anti-CYR61/CCN1 antibody [EPR20681]
    See all CYR61/CCN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20681] to CYR61/CCN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CYR61/CCN1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O00622

  • Positive control

    • WB: PC-3, MDA-MB-231 and Saos-2 whole cell lysate. ICC/IF: MDA-MB-231 cells. Flow Cytometry: MDA-MB-231 cells. IP: Saos-2 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 42 kDa.
ICC/IF 1/500.
Flow Cyt 1/60.
IP 1/30.

Target

  • Function

    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3CH61 antibody
    • CCN 1 antibody
    • CCN family member 1 antibody
    • CCN1 antibody
    • CYR 61 antibody
    • Cyr61 antibody
    • CYR61 protein antibody
    • CYR61_HUMAN antibody
    • Cysteine rich angiogenic inducer 61 antibody
    • Cysteine rich heparin binding protein 61 antibody
    • Cysteine-rich angiogenic inducer 61 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • IBP-10 antibody
    • IGF-binding protein 10 antibody
    • Igfbp 10 antibody
    • IGFBP-10 antibody
    • Igfbp10 antibody
    • Insulin like growth factor binding protein 10 antibody
    • Insulin-like growth factor-binding protein 10 antibody
    • Protein CYR61 antibody
    • Protein GIG1 antibody
    see all

Images

  • Anti-CYR61/CCN1 antibody [EPR20681] (ab230947) at 1/5000 dilution + MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/ Dilution buffer concentration: 5% NFDM/TBST.

    The molecular mass observed is consistent with that of the full-length protein (42 kDa) (PMID: 23798676).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labeling CYR61/CCN1 with ab230947 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the MDA-MB-231 cell line. Negative control: MCF7 (PMID: 11059746). The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

  • CYR61/CCN1 was immunoprecipitated from 0.35 mg Saos-2 (human osteosarcoma epithelial) whole cell lysate with ab230947 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: Saos-2 (human osteosarcoma epithelial) whole cell lysate 10 µg (Input).
    Lane 2: ab230947 IP in Saos-2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230947 in Saos-2 whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 8 seconds.

    The molecular mass observed is consistent with that of the full-length protein (42 kDa) (PMID: 23798676).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / MDA-MB-231 (human breast adenocarcinoma epithelial cell, Right) cell line labeling CYR61/CCN1 with ab230947 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: MCF7 (PMID: 11059746).

  • All lanes : Anti-CYR61/CCN1 antibody [EPR20681] (ab230947) at 1/1000 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Saos-2 (human osteosarcoma epithelial) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with the literature, with the 42 kDa band being the full-length protein and the 19 kDa band likely a degradation product (PMID: 23798676; PMID: 16266990; PMID: 8657105).

    Negative control: MCF7 (PMID 11059746).

References

ab230947 has not yet been referenced specifically in any publications.

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