Overview

  • Product name

    Anti-CysLT1 antibody - N-terminal
    See all CysLT1 primary antibodies
  • Description

    Rabbit polyclonal to CysLT1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N-terminal amino acids 1-45 of Human CysLT1 (Uniprot ID:Q9Y271).

  • Positive control

    • 293T, HeLa S3 and HepG2 whole cell lysates, U373 xenograft

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.
  • Tissue specificity

    Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLTR1_HUMAN antibody
    • CYSLT 1 antibody
    • CysLT(1) antibody
    • CYSLT1R antibody
    • CYSLTR 1 antibody
    • CYSLTR antibody
    • CysLTR vide supra antibody
    • CysLTR1 antibody
    • Cysteinyl leukotriene D4 receptor antibody
    • Cysteinyl leukotriene receptor 1 antibody
    • G-protein coupled receptor HG55 antibody
    • HG55 antibody
    • HMTMF81 antibody
    • LTD4 receptor antibody
    • MGC46139 antibody
    see all

Images

  • Anti-CysLT1 antibody - N-terminal (ab151484) at 1/500 dilution + 293T whole cell
    lysate at 30 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded U373 xenograft labeling CysLT1 with ab151484 antibody at 1/100.

References

This product has been referenced in:

  • Butler CT  et al. A Quininib Analogue and Cysteinyl Leukotriene Receptor Antagonist Inhibits Vascular Endothelial Growth Factor (VEGF)-independent Angiogenesis and Exerts an Additive Antiangiogenic Response with Bevacizumab. J Biol Chem 292:3552-3567 (2017). Read more (PubMed: 28035003) »
  • Reynolds AL  et al. Phenotype-based Discovery of 2-[(E)-2-(Quinolin-2-yl)vinyl]phenol as a Novel Regulator of Ocular Angiogenesis. J Biol Chem 291:7242-55 (2016). Read more (PubMed: 26846851) »
See all 2 Publications for this product

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