Anti-CysLT1 antibody - N-terminal (ab151484)
Key features and details
- Rabbit polyclonal to CysLT1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CysLT1 antibody - N-terminal -
Description
Rabbit polyclonal to CysLT1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within N-terminal amino acids 1-45 of Human CysLT1 (Uniprot ID:Q9Y271).
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Positive control
- 293T, HeLa S3 and HepG2 whole cell lysates, U373 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4. -
Tissue specificity
Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10800 Human
- Omim: 300201 Human
- SwissProt: Q9Y271 Human
- Unigene: 201300 Human
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Alternative names
- CLTR1_HUMAN antibody
- CYSLT 1 antibody
- CysLT(1) antibody
see all
Images
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Anti-CysLT1 antibody - N-terminal (ab151484) diluted at 1/500. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Immunohistochemical analysis of paraffin embedded U373 xenograft labeling CysLT1 with ab151484 antibody at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab151484 has been referenced in 6 publications.
- Koller A et al. Chronobiological activity of cysteinyl leukotriene receptor 1 during basal and induced autophagy in the ARPE-19 retinal pigment epithelial cell line. Aging (Albany NY) 13:25670-25693 (2021). PubMed: 34919533
- Koller A et al. Cysteinyl leukotriene receptor 1 modulates autophagic activity in retinal pigment epithelial cells. Sci Rep 10:17659 (2020). WB, ICC ; Human . PubMed: 33077798
- Slater K et al. High Cysteinyl Leukotriene Receptor 1 Expression Correlates with Poor Survival of Uveal Melanoma Patients and Cognate Antagonist Drugs Modulate the Growth, Cancer Secretome, and Metabolism of Uveal Melanoma Cells. Cancers (Basel) 12:N/A (2020). PubMed: 33066024
- Lin Y et al. Tetrandrine Ameliorates Airway Remodeling of Chronic Asthma by Interfering TGF-ß1/Nrf-2/HO-1 Signaling Pathway-Mediated Oxidative Stress. Can Respir J 2019:7930396 (2019). PubMed: 31781316
- Butler CT et al. A Quininib Analogue and Cysteinyl Leukotriene Receptor Antagonist Inhibits Vascular Endothelial Growth Factor (VEGF)-independent Angiogenesis and Exerts an Additive Antiangiogenic Response with Bevacizumab. J Biol Chem 292:3552-3567 (2017). PubMed: 28035003
- Reynolds AL et al. Phenotype-based Discovery of 2-[(E)-2-(Quinolin-2-yl)vinyl]phenol as a Novel Regulator of Ocular Angiogenesis. J Biol Chem 291:7242-55 (2016). PubMed: 26846851