Overview

  • Product name

    Anti-Cystathionase/CTH antibody
    See all Cystathionase/CTH primary antibodies
  • Description

    Rabbit polyclonal to Cystathionase/CTH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Cystathionase/CTH (internal sequence).
    (Peptide available as ab183695)

  • Positive control

    • SMMC cells; Rat liver tissue.
  • General notes

     This product was previously labelled as Cystathionase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab131052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure.
  • Pathway

    Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.
  • Involvement in disease

    Defects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.
  • Sequence similarities

    Belongs to the trans-sulfuration enzymes family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CGL_HUMAN antibody
    • CTH antibody
    • cystathionase (cystathionine gamma-lyase) antibody
    • Cystathionine gamma lyase antibody
    • Cystathionine gamma-lyase antibody
    • Cysteine desulfhydrase antibody
    • Gamma cystathionase antibody
    • Gamma-cystathionase antibody
    • Homoserine deaminase antibody
    • Homoserine dehydratase antibody
    • MGC9471 antibody
    see all

Images

  • Cystathionase/CTH was detected in frozen section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab131052 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Cystathionase/CTH was detected in frozen section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab131052 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • All lanes : Anti-Cystathionase/CTH antibody (ab131052) at 0.5 µg/ml

    Lane 1 : rat liver tissue lysates
    Lane 2 : rat kidney tissue lysates
    Lane 3 : SMMC-7721 whole cell lysates
    Lane 4 : A431 whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemical analysis of SMMC Cells labelled with ab131052 at 1 µg/ml.
  • Immunohistochemical analysis of paraffin embedded Rat Liver Tissue labelled with ab131052 at 1 µg/ml.

References

This product has been referenced in:

  • Marino A  et al. The novel H2 S donor 4-carboxy-phenyl isothiocyanate inhibits mast cell degranulation and renin release by decreasing intracellular calcium. Br J Pharmacol 173:3222-3234 (2016). Read more (PubMed: 27548075) »
See 1 Publication for this product

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