Anti-Cystathionase/CTH antibody (ab151769)
Key features and details
- Rabbit polyclonal to Cystathionase/CTH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cystathionase/CTH antibody
See all Cystathionase/CTH primary antibodies -
Description
Rabbit polyclonal to Cystathionase/CTH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Cystathionase/CTH aa 194-405.
Database link: P32929 -
Positive control
- HeLa, MOLT4 and mouse liver whole cell lysates, hepatoma tissue
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General notes
This product was previously labelled as Cystathionase
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. -
Pathway
Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2. -
Involvement in disease
Defects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. -
Sequence similarities
Belongs to the trans-sulfuration enzymes family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1491 Human
- Entrez Gene: 107869 Mouse
- Omim: 607657 Human
- SwissProt: P32929 Human
- SwissProt: Q8VCN5 Mouse
- Unigene: 19904 Human
- Unigene: 28301 Mouse
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Alternative names
- CGL_HUMAN antibody
- CTH antibody
- cystathionase (cystathionine gamma-lyase) antibody
see all
Images
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Western blot - Anti-Cystathionase/CTH antibody (ab151769)All lanes : Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution
Lane 1 : HeLa whole cell
lysate
Lane 2 : MOLT4 whole cell
lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Western blot - Anti-Cystathionase/CTH antibody (ab151769)Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystathionase/CTH antibody (ab151769)Immunohistochemical analysis of paraffin embedded human hepatoma tissue labeling Cystathionase/CTH with ab151769 antibody at 1/500.
Protocols
References (13)
ab151769 has been referenced in 13 publications.
- Wu L et al. H2S-activatable near-infrared afterglow luminescent probes for sensitive molecular imaging in vivo. Nat Commun 11:446 (2020). PubMed: 31974383
- Augsburger F et al. Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics in Murine Colon Cancer Cells. Biomolecules 10:N/A (2020). PubMed: 32183148
- Wilkie SE et al. Strain-specificity in the hydrogen sulphide signalling network following dietary restriction in recombinant inbred mice. Geroscience 42:801-812 (2020). PubMed: 32162209
- Nlandu-Khodo S et al. Tubular ß-catenin and FoxO3 interactions protect in chronic kidney disease. JCI Insight 5:N/A (2020). PubMed: 32369448
- Wang SY et al. Methionine restriction delays senescence and suppresses the senescence-associated secretory phenotype in the kidney through endogenous hydrogen sulfide. Cell Cycle 18:1573-1587 (2019). PubMed: 31164038
