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  1. Link

    cystathionasecth-antibody-ab151769.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-Cystathionase/CTH antibody (ab151769)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (13)

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Western blot - Anti-Cystathionase/CTH antibody (ab151769)
  • Western blot - Anti-Cystathionase/CTH antibody (ab151769)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystathionase/CTH antibody (ab151769)

Key features and details

  • Rabbit polyclonal to Cystathionase/CTH
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cystathionase/CTH antibody
    See all Cystathionase/CTH primary antibodies
  • Description

    Rabbit polyclonal to Cystathionase/CTH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Cystathionase/CTH aa 194-405.
    Database link: P32929

  • Positive control

    • HeLa, MOLT4 and mouse liver whole cell lysates, hepatoma tissue
  • General notes

     This product was previously labelled as Cystathionase

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Mouse liver tissue lysate - total protein (ab29301)
    • HeLa whole cell lysate (ab29545)
    • MOLT4 whole cell lysate (ab7912)
  • Related Products

    • Recombinant Human Cystathionase/CTH protein (ab123201)

Applications

Our Abpromise guarantee covers the use of ab151769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure.
  • Pathway

    Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.
  • Involvement in disease

    Defects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.
  • Sequence similarities

    Belongs to the trans-sulfuration enzymes family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P32929 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1491 Human
    • Entrez Gene: 107869 Mouse
    • Omim: 607657 Human
    • SwissProt: P32929 Human
    • SwissProt: Q8VCN5 Mouse
    • Unigene: 19904 Human
    • Unigene: 28301 Mouse
    • Alternative names

      • CGL_HUMAN antibody
      • CTH antibody
      • cystathionase (cystathionine gamma-lyase) antibody
      • Cystathionine gamma lyase antibody
      • Cystathionine gamma-lyase antibody
      • Cysteine desulfhydrase antibody
      • Gamma cystathionase antibody
      • Gamma-cystathionase antibody
      • Homoserine deaminase antibody
      • Homoserine dehydratase antibody
      • MGC9471 antibody
      see all

    Images

    • Western blot - Anti-Cystathionase/CTH antibody (ab151769)
      Western blot - Anti-Cystathionase/CTH antibody (ab151769)
      All lanes : Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution

      Lane 1 : HeLa whole cell
      lysate
      Lane 2 : MOLT4 whole cell
      lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 45 kDa



      10% SDS PAGE
    • Western blot - Anti-Cystathionase/CTH antibody (ab151769)
      Western blot - Anti-Cystathionase/CTH antibody (ab151769)
      Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

      Predicted band size: 45 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystathionase/CTH antibody (ab151769)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystathionase/CTH antibody (ab151769)

      Immunohistochemical analysis of paraffin embedded human hepatoma tissue labeling Cystathionase/CTH with ab151769 antibody at 1/500.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (13)

    Publishing research using ab151769? Please let us know so that we can cite the reference in this datasheet.

    ab151769 has been referenced in 13 publications.

    • Wu L  et al. H2S-activatable near-infrared afterglow luminescent probes for sensitive molecular imaging in vivo. Nat Commun 11:446 (2020). PubMed: 31974383
    • Augsburger F  et al. Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics in Murine Colon Cancer Cells. Biomolecules 10:N/A (2020). PubMed: 32183148
    • Wilkie SE  et al. Strain-specificity in the hydrogen sulphide signalling network following dietary restriction in recombinant inbred mice. Geroscience 42:801-812 (2020). PubMed: 32162209
    • Nlandu-Khodo S  et al. Tubular ß-catenin and FoxO3 interactions protect in chronic kidney disease. JCI Insight 5:N/A (2020). PubMed: 32369448
    • Wang SY  et al. Methionine restriction delays senescence and suppresses the senescence-associated secretory phenotype in the kidney through endogenous hydrogen sulfide. Cell Cycle 18:1573-1587 (2019). PubMed: 31164038
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Cystathionase/CTH antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient gel)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 31 2017

    Question

    No signal detected in adipose tissue, strong signal in kidney and liver tissue.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2014

    Answer

    Below is a link to the human protein atlas file for Cystathionase. As you can see, adipose tissue did not show any signal and very low RNA expression, while liver and kidney tissue gave very stong signal. Thus, I would not expect consistent results in fat tissue, and you may need to load a large amount of protein and use a high antibody concentration to yield any positive signal:

    http://www.proteinatlas.org/ENSG00000116761/tissue

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Feb 20 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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