Anti-Cystathionase/CTH antibody (ab151769)
- Datasheet
- References (8)
- Protocols
Overview
-
Product name
Anti-Cystathionase/CTH antibody
See all Cystathionase/CTH primary antibodies -
Description
Rabbit polyclonal to Cystathionase/CTH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Cystathionase/CTH aa 194-405.
Database link: P32929 -
Positive control
- HeLa, MOLT4 and mouse liver whole cell lysates, hepatoma tissue
-
General notes
This product was previously labelled as Cystathionase
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab151769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa.Can be blocked with Recombinant Human Cystathionase/CTH protein (ab114582). | |
| IHC-P | 1/100 - 1/1000. |
Target
-
Function
Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. -
Pathway
Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2. -
Involvement in disease
Defects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. -
Sequence similarities
Belongs to the trans-sulfuration enzymes family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 1491 Human
- Entrez Gene: 107869 Mouse
- Omim: 607657 Human
- SwissProt: P32929 Human
- SwissProt: Q8VCN5 Mouse
- Unigene: 19904 Human
- Unigene: 28301 Mouse
-
Alternative names
- CGL_HUMAN antibody
- CTH antibody
- cystathionase (cystathionine gamma-lyase) antibody
see all
Images
-
Western blot - Anti-Cystathionase/CTH antibody (ab151769)All lanes : Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution
Lane 1 : HeLa whole cell
lysate
Lane 2 : MOLT4 whole cell
lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Western blot - Anti-Cystathionase/CTH antibody (ab151769)Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystathionase/CTH antibody (ab151769)Immunohistochemical analysis of paraffin embedded human hepatoma tissue labeling Cystathionase/CTH with ab151769 antibody at 1/500.
References
This product has been referenced in:
- Luo R et al. Hydrogen sulfide upregulates renal AQP-2 protein expression and promotes urine concentration. FASEB J 33:469-483 (2019). Read more (PubMed: 30036087) »
- Yang J et al. Non-enzymatic hydrogen sulfide production from cysteine in blood is catalyzed by iron and vitamin B6. Commun Biol 2:194 (2019). Read more (PubMed: 31123718) »
