Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15468] to Cystathionase/CTH - N-terminal
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Product nameAnti-Cystathionase/CTH antibody [EPR15468] - N-terminal
See all Cystathionase/CTH primary antibodies
DescriptionRabbit monoclonal [EPR15468] to Cystathionase/CTH - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cystathionase/CTH aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P32929
- HeLa and K562 cell lysates.
This product was previously labelled as Cystathionase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab189916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IP||1/50 - 1/70.|
FunctionCatalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure.
PathwayAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.
Involvement in diseaseDefects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.
Sequence similaritiesBelongs to the trans-sulfuration enzymes family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CGL_HUMAN antibody
- CTH antibody
- cystathionase (cystathionine gamma-lyase) antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Cystathionase/CTH knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab189916 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab189916 was shown to recognize Cystathionase/CTH in wild type cells as signal was lost at the expected MW in Cystathionase/CTH knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Cystathionase/CTH knockout samples were subjected to SDS-PAGE. Ab189916 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Anti-Cystathionase/CTH antibody [EPR15468] - N-terminal (ab189916) at 0.01 µg/ml + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 45 kDa
Exposure time: 1 minute
Blocking and diluting buffer: 5% NFDM/TBST.
Immunoprecipitation of Cystathionase/CTH from HeLa cell lysate using ab189916 at 1/50 or 1X PBS (negative control) (lane 1). Immunostained with ab189916 at 1/1500 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab189916 has been referenced in 1 publication.
- Fan J et al. Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine ? lyase S-sulfhydration in ApoE-knockout atherosclerotic mice. Br J Pharmacol 176:3180-3192 (2019). PubMed: 31140595