Anti-Cystatin A antibody [WR-23/2/3/3] (ab10442)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-Cystatin A antibody [WR-23/2/3/3]
See all Cystatin A primary antibodies -
DescriptionMouse monoclonal [WR-23/2/3/3] to Cystatin A
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Full length protein (Human).
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Positive control
- Human Thymus tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 15mM Sodium Azide
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberWR-23/2/3/3
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab10442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 11-13 kDa. |
Target
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FunctionThis is an intracellular thiol proteinase inhibitor.
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Sequence similaritiesBelongs to the cystatin family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 1475 Human
- Entrez Gene: 209294 Mouse
- Omim: 184600 Human
- SwissProt: P01040 Human
- SwissProt: P56567 Mouse
- Unigene: 518198 Human
- Unigene: 300592 Mouse
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Alternative names
- AREI antibody
- cstA antibody
- Cystatin A antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin A antibody [WR-23/2/3/3] (ab10442)](https://www.abcam.com/ps/products/10/ab10442/Images/Cystatin-A-Primary-antibodies-ab10442-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin A antibody [WR-23/2/3/3] (ab10442)IHC image of ab10442 staining in normal human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References
This product has been referenced in:
- Lundberg KC et al. Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans. Mol Cell Proteomics 14:109-19 (2015). Read more (PubMed: 25351201) »
- Tang M et al. Expression and Prognostic Significance of Macrophage Inflammatory Protein-3 Alpha and Cystatin A in Nasopharyngeal Carcinoma. Biomed Res Int 2015:617143 (2015). IHC . Read more (PubMed: 26634210) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"