Overview

  • Product name

    Anti-Cystatin-B antibody [2F1]
    See all Cystatin-B primary antibodies
  • Description

    Mouse monoclonal [2F1] to Cystatin-B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Cystatin-B.

  • Positive control

    • A549 cell lysate
  • General notes

    Cystatin-B is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cystatin-B protein.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

    This product was previously labelled as Stefin B.

    This product was changed from ascites to tissue culture supernatant on 08/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab72353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ELISA Use at an assay dependent concentration.

Target

  • Function

    This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
  • Involvement in disease

    Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.
  • Sequence similarities

    Belongs to the cystatin family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHROW21 antibody
    • CPI B antibody
    • CPI-B antibody
    • CST 6 antibody
    • CST6 antibody
    • CSTB antibody
    • Cystatin B (stefin B) antibody
    • Cystatin B antibody
    • Cystatin-B antibody
    • CYTB antibody
    • CYTB_HUMAN antibody
    • EPM1 antibody
    • EPM1A antibody
    • Liver thiol proteinase inhibitor antibody
    • PME antibody
    • Stefin-B antibody
    • STF B antibody
    • STFB antibody
    • ULD antibody
    see all

Images

  • ICC/IF analysis of CSTB in A549 cells. The cell was stained with ab72353(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

  • All lanes : Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : U87MG cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 11 kDa

  • Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Burgener A  et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . Read more (PubMed: 23449785) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
hippocampus
Blocking step
BSA as blocking agent for 9 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Paige Mumford

Verified customer

Submitted Jul 06 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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