Key features and details
- Mouse monoclonal [2F1] to Cystatin-B
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Cystatin-B antibody [2F1]
See all Cystatin-B primary antibodies
DescriptionMouse monoclonal [2F1] to Cystatin-B
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Cystatin-B.
- A549 cell lysate
Cystatin-B is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cystatin-B protein.
This product was previously labelled as Stefin B.
This product was changed from ascites to tissue culture supernatant on 08/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab72353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).|
|ELISA||Use at an assay dependent concentration.|
FunctionThis is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Involvement in diseaseDefects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.
Sequence similaritiesBelongs to the cystatin family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CHROW21 antibody
- CPI B antibody
- CPI-B antibody
ICC/IF analysis of CSTB in A549 cells. The cell was stained with ab72353(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
All lanes : Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : U87MG cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 11 kDa
Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg
goat anti-mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
This image was generated using the ascites version of the product.
ab72353 has been referenced in 1 publication.
- Burgener A et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . PubMed: 23449785