Product nameAnti-Cystatin-B antibody [EPR3931]
See all Cystatin-B primary antibodies
DescriptionRabbit monoclonal [EPR3931] to Cystatin-B
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Cystatin-B aa 50-150. The exact sequence is proprietary.
- U87-MG, JAR, and Human bladder cell lysates; Human colon tissue; Human kidney tissue
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Stefin B
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.
WB: 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Involvement in diseaseDefects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.
Sequence similaritiesBelongs to the cystatin family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CHROW21 antibody
- CPI B antibody
- CPI-B antibody
All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Human bladder lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human colon tissue.
ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human kidney tissue.
ab92449 has not yet been referenced specifically in any publications.