Recombinant Anti-Cystatin-B antibody [EPR3931] (ab92449)


  • Product name

    Anti-Cystatin-B antibody [EPR3931]
    See all Cystatin-B primary antibodies
  • Description

    Rabbit monoclonal [EPR3931] to Cystatin-B
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Cystatin-B aa 50-150. The exact sequence is proprietary.

  • Positive control

    • U87-MG, JAR, and Human bladder cell lysates; Human colon tissue; Human kidney tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


     This product was previously labelled as Stefin B


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab92449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: 1/10 - 1/100.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.

    Is unsuitable for ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
    • Involvement in disease

      Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.
    • Sequence similarities

      Belongs to the cystatin family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CHROW21 antibody
      • CPI B antibody
      • CPI-B antibody
      • CST 6 antibody
      • CST6 antibody
      • CSTB antibody
      • Cystatin B (stefin B) antibody
      • Cystatin B antibody
      • Cystatin-B antibody
      • CYTB antibody
      • CYTB_HUMAN antibody
      • EPM1 antibody
      • EPM1A antibody
      • Liver thiol proteinase inhibitor antibody
      • PME antibody
      • Stefin-B antibody
      • STF B antibody
      • STFB antibody
      • ULD antibody
      see all


    • All lanes : Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : Human bladder lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 11 kDa

    • ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human colon tissue.

    • ab92449, at 1/100 dilution, staining Cystatin-B in paraffin-embedded Human kidney tissue.


    ab92449 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for your reply and for spotting my mistake. ab92449 is indeed described as not suitable for mouse samples. So, as requested, I have issued a free of charge replacement with ab54566. The order number is 954882. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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    Thank you for your reply and those precisions. We have an alternative anti-CSTB, ab92449, directed against the C-terminal region of the human protein (between amino acids 73 and 98). The homology between the region where ab92449 binds and the mouse CSTB is 85%. Would you like to test this antibody on Mouse samples? If so, I will be happy to send you this antibody as a replacement of ab53725.

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