Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cystatin C antibody [EPR4413] (ab109508)

Reviews (1) Submit a question References (13)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4413] to Cystatin C
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cystatin C antibody [EPR4413]
    See all Cystatin C primary antibodies

  • Description

    Rabbit monoclonal [EPR4413] to Cystatin C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant protein fragment corresponding to residues in Human Cystatin C.

  • Positive control

    • WB: Human testis, Caco2, U20S, HeLa, HepG2, and U87-MG lysates. IHC-P: Human brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

    • Tissue specificity

      Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

    • Involvement in disease

      Amyloidosis 6
      Macular degeneration, age-related, 11

    • Sequence similarities

      Belongs to the cystatin family.

    • Post-translational
      modifications

      The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AD 8 antibody
      • AD8 antibody
      • Amyloid angiopathy and cerebral hemorrhage antibody
      • ARMD11 antibody
      • bA218C14.4 (cystatin C) antibody
      • bA218C14.4 antibody
      • Cst 3 antibody
      • Cst3 antibody
      • CST3 protein antibody
      • Cystatin 3 antibody
      • Cystatin-3 antibody
      • Cystatin-C antibody
      • Cystatin3 antibody
      • CystatinC antibody
      • CYTC_HUMAN antibody
      • Epididymis secretory protein Li 2 antibody
      • Gamma trace antibody
      • Gamma-trace antibody
      • HCCAA antibody
      • HEL S 2 antibody
      • MGC117328 antibody
      • Neuroendocrine basic polypeptide antibody
      • Post gamma globulin antibody
      • Post-gamma-globulin antibody
      see all

    Images

    • Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
      Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: CST3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Caco2 whole cell lysate (20 µg)
      Lane 4: U20S whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109508 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. Ab109508 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
      Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
      All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 15 kDa
      Observed band size: 13 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Cystatin C antibody [EPR4413] (ab109508)
      Anti-Cystatin C antibody [EPR4413] (ab109508)

    References (13)

    Publishing research using ab109508? Please let us know so that we can cite the reference in this datasheet.

    ab109508 has been referenced in 13 publications.

    • Grigore F  et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases. Neoplasia 22:376-389 (2020). PubMed: 32629178
    • Kogot-Levin A  et al. Proximal Tubule mTORC1 Is a Central Player in the Pathophysiology of Diabetic Nephropathy and Its Correction by SGLT2 Inhibitors. Cell Rep 32:107954 (2020). PubMed: 32726619
    • Sher AA  et al. Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance. Front Microbiol 10:596 (2019). PubMed: 30984137
    • Watanabe S  et al. Intracerebroventricular administration of Cystatin C ameliorates disease in SOD1-linked amyotrophic lateral sclerosis mice. J Neurochem 145:80-89 (2018). PubMed: 29282717
    • Lee RK  et al. Expression of cystatin C in the female reproductive tract and its effect on human sperm capacitation. Reprod Biol Endocrinol 16:8 (2018). PubMed: 29378615
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Cystatin C antibody [EPR4413]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com