Recombinant Anti-Cystatin C antibody [EPR4413] (ab109508)
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-283569-anti-cystatin-c-antibody-epr4413-western-blot.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4413] to Cystatin C
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cystatin C antibody [EPR4413]
See all Cystatin C primary antibodies -
Description
Rabbit monoclonal [EPR4413] to Cystatin C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis, Caco2, U20S, HeLa, HepG2, and U87-MG lysates, rat heart tissue lysate and mouse thymus tissue lysate. IHC-P: Human brain and kidney tissue, mouse kidney tissue and rat kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4413 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
|
| IHC-P |
1/250 - 1/8000.
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| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). |
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IHC-P
1/250 - 1/8000. |
Target
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Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
Involvement in disease
Amyloidosis 6
Macular degeneration, age-related, 11 -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1471 Human
- Entrez Gene: 13010 Mouse
- Entrez Gene: 25307 Rat
- Omim: 604312 Human
- SwissProt: P01034 Human
- SwissProt: P21460 Mouse
- SwissProt: P14841 Rat
- Unigene: 304682 Human
see all -
Alternative names
- AD 8 antibody
- AD8 antibody
- Amyloid angiopathy and cerebral hemorrhage antibody
see all
Images
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Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CST3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Caco2 whole cell lysate (20 µg)
Lane 4: U20S whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109508 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. Ab109508 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-489124-anti-cystatin-c-antibody-epr4413-immunohistochemistry-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)Immunohistochemistry analysis of paraffin-embedded human kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on human kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-489122-anti-cystatin-c-antibody-epr4413-western-blot-mouse.jpg)
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution + Mouse thymus tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-489128-anti-cystatin-c-antibody-epr4413-immunohistochemistry-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)Immunohistochemistry analysis of paraffin-embedded rat kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on rat kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-489126-anti-cystatin-c-antibody-epr4413-immunohistochemistry-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on mouse kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-489120-anti-cystatin-c-antibody-epr4413-western-blot-rat.jpg)
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution + Rat heart tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Exposure time: 80 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST -
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-122414-anti-cystatin-c-antibody-epr4413-western-blot.jpg)
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution
Lane 1 : Human testis lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-122413-anti-cystatin-c-antibody-epr4413-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Cystatin C antibody [EPR4413] (ab109508)](https://www.abcam.com/ps/products/109/ab109508/Images/ab109508-4-benefits-of-recombinant-antibodies.png)
Anti-Cystatin C antibody [EPR4413] (ab109508)
Protocols
Datasheets and documents
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Datasheet download
References (22)
ab109508 has been referenced in 22 publications.
- Indacochea A et al. Cold-inducible RNA binding protein promotes breast cancer cell malignancy by regulating Cystatin C levels. RNA 27:190-201 (2021). PubMed: 33172965
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Shen Y et al. Pressure overload promotes cystatin C secretion of cardiomyocytes to regulate the MAPK signaling pathway and mediate cardiac hypertrophy. Ann Transl Med 8:1514 (2020). PubMed: 33313259
- Grigore F et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases. Neoplasia 22:376-389 (2020). PubMed: 32629178
- Kogot-Levin A et al. Proximal Tubule mTORC1 Is a Central Player in the Pathophysiology of Diabetic Nephropathy and Its Correction by SGLT2 Inhibitors. Cell Rep 32:107954 (2020). PubMed: 32726619
