Overview

  • Product name

    Cysteinyl leukotriene ELISA kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 295pg/ml 4.6%
    Medium 544pg/ml 4.7%
    High 1036pg/ml 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Low 253pg/ml 4.6%
    Medium 617pg/ml 6.2%
    High 941pg/ml 8.3%
  • Sample type

    Urine, Tissue Culture Media
  • Assay type

    Competitive
  • Sensitivity

    26.6 pg/ml
  • Range

    78.1 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 107.6 % - %
    Tissue Culture Media 101 1% - 0.5%

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Cysteinyl Leukotriene in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cysteinyl Leukotriene in Human Urine and Tissue Culture Media.

    A goat anti-mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Cysteinyl Leukotriene antigen and a monoclonal mouse antibody specific to Cysteinyl Leukotriene. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Cysteinyl Leukotriene captured in the plate.

  • Notes

    Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.

    Cross Reactivity

    Compound% Cross Reactivity
    LTC4100
    LTD4115.12
    LTE462.74
    LTB41.16
    Arachadonic Acid0.61
    PGE20.48
    6-trans-LTB40.45
    TXB20.28
    20-OH-LTB4<0.2
    PGF<0.2
    6-keto-PGF<0.2
    PGD2<0.2
    15-HETE<0.2
    12-HETE<0.2
    15-HETE<0.2
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133042.

Protocols

References

ab133042 has not yet been referenced specifically in any publications.

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