Recombinant Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8178] to Cytochrome b-c1 complex subunit 9/UQCR10
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178]
See all Cytochrome b-c1 complex subunit 9/UQCR10 primary antibodies -
Description
Rabbit monoclonal [EPR8178] to Cytochrome b-c1 complex subunit 9/UQCR10 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, Jurkat, Saos-2 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8178 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/20 - 1/40.
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WB |
1/1000 - 1/10000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa).
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Notes |
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IP
1/20 - 1/40. |
WB
1/1000 - 1/10000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa). |
Target
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Relevance
Function: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1. Similarity: Belongs to the UQCR10/QCR9 family. -
Cellular localization
Mitochondrial; Mitochondrion inner membrane -
Database links
- Entrez Gene: 29796 Human
- Entrez Gene: 66152 Mouse
- Omim: 610843 Human
- SwissProt: Q9UDW1 Human
- SwissProt: Q8R1I1 Mouse
- Unigene: 284292 Human
- Unigene: 269736 Mouse
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Alternative names
- Cytochrome c1 non heme 7 kDa protein antibody
- Cytochrome C1 nonheme 7kDa protein antibody
- HSPC051 antibody
see all
Images
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Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154) at 1/5000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa -
All lanes : Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa -
Western blot analysis of Cytochrome b-c1 complex subunit 9/UQCR10 in HepG2 cell lysate immunoprecipitated with ab181154 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181154 has not yet been referenced specifically in any publications.