Recombinant Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8179] to Cytochrome b-c1 complex subunit 9/UQCR10
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179]
See all Cytochrome b-c1 complex subunit 9/UQCR10 primary antibodies -
Description
Rabbit monoclonal [EPR8179] to Cytochrome b-c1 complex subunit 9/UQCR10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cytochrome b-c1 complex subunit 9/UQCR10 aa 50-150. The exact sequence is proprietary.
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Positive control
- MCF7, HepG2, HeLa, Human fetal heart, and Human skeletal muscle lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Cytochrome b-c1 complex subunit 9
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 3.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8179 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab134909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 7 kDa. | |
| ICC/IF | 1/250 - 1/500. |
Target
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Relevance
Function: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1. Similarity: Belongs to the UQCR10/QCR9 family. -
Cellular localization
Mitochondrial; Mitochondrion inner membrane -
Database links
- Entrez Gene: 29796 Human
- Omim: 610843 Human
- SwissProt: Q9UDW1 Human
- Unigene: 284292 Human
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Alternative names
- Cytochrome c1 non heme 7 kDa protein antibody
- Cytochrome C1 nonheme 7kDa protein antibody
- HSPC051 antibody
see all
Images
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![Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)](https://www.abcam.com/ps/products/134/ab134909/Images/ab134909-149038-anti-cytochrome-b-c1-complex-subunit-9-antibody-epr8179-western-blot.jpg)
Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)All lanes : Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human fetal heart lysate
Lane 5 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 7 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)](https://www.abcam.com/ps/products/134/ab134909/Images/ab134909-149037-anti-cytochrome-b-c1-complex-subunit-9-antibody-epr8179-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)Immunofluorescent analysis of HeLa cells labelling Cytochrome b-c1 complex subunit 9/UQCR10 with ab134909 at 1/250 dilution.
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![OI-RD Scanning - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)](https://www.abcam.com/ps/products/134/ab134909/Images/ab134909-149036-anti-cytochrome-b-c1-complex-subunit-9-antibody-epr8179.jpg)
OI-RD Scanning - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909) -
![Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)](https://www.abcam.com/ps/products/134/ab134909/Images/ab134909-4-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (ab134909)
Protocols
References (1)
ab134909 has been referenced in 1 publication.
- Daniel HD & Torbenson M Transgenic hepatitis B: a new model of HBV infection. Sci Rep 7:2610 (2017). PubMed: 28572637
