Product nameAnti-Cytochrome b reductase 1/DCYTB antibody
See all Cytochrome b reductase 1/DCYTB primary antibodies
DescriptionRabbit polyclonal to Cytochrome b reductase 1/DCYTB
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Pig, Orangutan
Synthetic peptide corresponding to Mouse Cytochrome b reductase 1/DCYTB aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in A20 whole cell lysate. ICC/IF: HeLa cells.
This product was previously labelled as Cytochrome b reductase 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab66048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-Fr||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
Tissue specificityPresent in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
Sequence similaritiesContains 1 cytochrome b561 domain.
- Information by UniProt
- CYBR 1 antibody
- CYBR1 antibody
- CYBR1_HUMAN antibody
Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048) at 1 µg/ml +
A20 whole cell lysate (ab7180) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 10 minutes
IHC-Fr image of human duodenum section stained with ab66048. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab66048, 1µg/ml) and Anti-Actin overnight at +4°C. The secondary antibody was Alexa Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. dCytb stained apical site of duodenum and actin stained muscles and blood vessels.
ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab66048 has not yet been referenced specifically in any publications.