Key features and details
- Rabbit polyclonal to Cytochrome b reductase 1/DCYTB
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cytochrome b reductase 1/DCYTB antibody
See all Cytochrome b reductase 1/DCYTB primary antibodies
DescriptionRabbit polyclonal to Cytochrome b reductase 1/DCYTB
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Pig, Orangutan
Synthetic peptide corresponding to Mouse Cytochrome b reductase 1/DCYTB aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
This product was previously labelled as Cytochrome b reductase 1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab66048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
Tissue specificityPresent in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
Sequence similaritiesContains 1 cytochrome b561 domain.
- Information by UniProt
- CYBR 1 antibody
- CYBR1 antibody
- CYBR1_HUMAN antibody
Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048) at 1 µg/ml +
A20 whole cell lysate (ab7180) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 10 minutes
ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab66048 has been referenced in 1 publication.
- Sherman HG et al. Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. Biochim Biophys Acta Bioenerg 1860:628-639 (2019). PubMed: 31229569