Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048)
Key features and details
- Rabbit polyclonal to Cytochrome b reductase 1/DCYTB
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome b reductase 1/DCYTB antibody
See all Cytochrome b reductase 1/DCYTB primary antibodies -
Description
Rabbit polyclonal to Cytochrome b reductase 1/DCYTB -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells. -
Tissue specificity
Present in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium. -
Sequence similarities
Contains 1 cytochrome b561 domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 79901 Human
- Entrez Gene: 73649 Mouse
- Entrez Gene: 295669 Rat
- Omim: 605745 Human
- SwissProt: Q53TN4 Human
- SwissProt: Q925G2 Mouse
- SwissProt: Q5RKJ2 Rat
- Unigene: 726027 Human
see all -
Alternative names
- CYBR 1 antibody
- CYBR1 antibody
- CYBR1_HUMAN antibody
see all
Images
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Anti-Cytochrome b reductase 1/DCYTB antibody (ab66048) at 1 µg/ml +
A20 whole cell lysate (ab7180) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 10 minutes -
ICC/IF image of ab66048 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66048, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab66048 has been referenced in 3 publications.
- Ma J et al. miR-423-3p activates FAK signaling pathway to drive EMT process and tumor growth in lung adenocarcinoma through targeting CYBRD1. J Clin Lab Anal 35:e24044 (2021). PubMed: 34714955
- Fang W et al. Molecular Hydrogen Protects Human Melanocytes from Oxidative Stress by Activating Nrf2 Signaling. J Invest Dermatol 140:2230-2241.e9 (2020). PubMed: 32234461
- Sherman HG et al. Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. Biochim Biophys Acta Bioenerg 1860:628-639 (2019). PubMed: 31229569