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Synthetic peptide corresponding to Cytochrome b245 Light Chain/p22-phox aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
Our Abpromise guarantee covers the use of ab75941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21 kDa).|
|IHC-P||Use a concentration of 1 µg/ml.|
We hypothesize that the 21 kDa band represents the pro-form of Cytochrome b245 Light Chain/p22-phox, and the 19 kDa band represents the mature form.
Immunohistochemical analyses of p22phox using ab75941 (1/200) in normal adrenocortical tissue (b-c) and adrenal aldosterone-producing adenoma, (e-f) p22phox presents a gradient of expression in the entire adrenal cortex (100x), and its localization was restricted to the cell membrane (400x). Sections were incubated with primary antibody at 4°C overnight and with a HRP-conjugated secondary antibody for 30 minutes at 37°C.
IHC image of Cytochrome b245 Light Chain/p22-phox staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75941, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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