Anti-Cytochrome b5 antibody (ab186863)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cytochrome b5 antibody
See all Cytochrome b5 primary antibodies -
Description
Rabbit polyclonal to Cytochrome b5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Cytochrome b5 aa 1-100 (N terminal).
Database link: P00167 -
Positive control
- MCF7, A549, HT29, 293T and M21 cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab186863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 15 kDa. | |
| IHC-P | 1/50 - 1/200. |
Target
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Function
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. -
Involvement in disease
Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. -
Sequence similarities
Belongs to the cytochrome b5 family.
Contains 1 cytochrome b5 heme-binding domain. -
Cellular localization
Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1528 Human
- Entrez Gene: 109672 Mouse
- Entrez Gene: 64001 Rat
- Omim: 613218 Human
- SwissProt: P00167 Human
- SwissProt: P56395 Mouse
- SwissProt: P00173 Rat
- Unigene: 465413 Human
see all -
Alternative names
- CYB 5 antibody
- CYB 5A antibody
- CYB5 antibody
see all
Images
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Western blot - Anti-Cytochrome b5 antibody (ab186863)All lanes : Anti-Cytochrome b5 antibody (ab186863) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : A549 cell extract
Lane 3 : HT29 cell extract
Lane 4 : 293T cell extract
Lane 5 : M21 cell extract
Predicted band size: 15 kDa
Datasheets and documents
References
ab186863 has not yet been referenced specifically in any publications.
