Key features and details
- Rabbit polyclonal to Cytochrome b5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cytochrome b5 antibody
See all Cytochrome b5 primary antibodies
DescriptionRabbit polyclonal to Cytochrome b5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Guinea pig, Cow, Dog, Rhesus monkey
Recombinant fragment within Human Cytochrome b5 (internal sequence). The exact sequence is proprietary.
Database link: P00167
- WB: Cytochrome b5-transfected HEK-293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 15 kDa.|
FunctionCytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Involvement in diseaseDefects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
Sequence similaritiesBelongs to the cytochrome b5 family.
Contains 1 cytochrome b5 heme-binding domain.
Cellular localizationCytoplasm and Endoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- CYB 5 antibody
- CYB 5A antibody
- CYB5 antibody
All lanes : Anti-Cytochrome b5 antibody (ab228751) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Cytochrome b5-transfected HEK-293T cell lysate
Developed using the ECL technique.
Predicted band size: 15 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228751 has not yet been referenced specifically in any publications.