Overview

  • Product name

    Anti-Cytochrome b5 antibody
    See all Cytochrome b5 primary antibodies
  • Description

    Rabbit polyclonal to Cytochrome b5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Guinea pig, Cow, Dog, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cytochrome b5 (internal sequence). The exact sequence is proprietary.
    Database link: P00167

  • Positive control

    • WB: Cytochrome b5-transfected HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 15 kDa.

Target

  • Function

    Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
  • Involvement in disease

    Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
  • Sequence similarities

    Belongs to the cytochrome b5 family.
    Contains 1 cytochrome b5 heme-binding domain.
  • Cellular localization

    Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CYB 5 antibody
    • CYB 5A antibody
    • CYB5 antibody
    • CYB5_HUMAN antibody
    • Cyb5a antibody
    • Cytochrome b 5 antibody
    • Cytochrome b5 (microsomal) antibody
    • Cytochrome b5 antibody
    • Cytochrome b5 type A (microsomal) antibody
    • Cytochrome b5 type A antibody
    • MCB 5 antibody
    • MCB5 antibody
    • Microsomal cytochrome b5 antibody
    • Microsomal cytochrome b5 type A antibody
    • Type 1 cyt b5 antibody
    see all

Images

  • All lanes : Anti-Cytochrome b5 antibody (ab228751) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : Cytochrome b5-transfected HEK-293T cell lysate

    Developed using the ECL technique.

    Predicted band size: 15 kDa



    12% SDS-PAGE

References

ab228751 has not yet been referenced specifically in any publications.

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