Overview

  • Product name
    Anti-Cytochrome b5 antibody
    See all Cytochrome b5 primary antibodies
  • Description
    Goat polyclonal to Cytochrome b5
  • Host species
    Goat
  • Specificity
    Non-specific cross-reactivity with 50 and 60 kDa species.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Human
  • Immunogen

    Full length native protein (purified) (Rabbit).

Properties

Applications

Our Abpromise guarantee covers the use of ab23852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 15 kDa.

Target

  • Function
    Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
  • Involvement in disease
    Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
  • Sequence similarities
    Belongs to the cytochrome b5 family.
    Contains 1 cytochrome b5 heme-binding domain.
  • Cellular localization
    Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CYB 5 antibody
      • CYB 5A antibody
      • CYB5 antibody
      • CYB5_HUMAN antibody
      • Cyb5a antibody
      • Cytochrome b 5 antibody
      • Cytochrome b5 (microsomal) antibody
      • Cytochrome b5 antibody
      • Cytochrome b5 type A (microsomal) antibody
      • Cytochrome b5 type A antibody
      • MCB 5 antibody
      • MCB5 antibody
      • Microsomal cytochrome b5 antibody
      • Microsomal cytochrome b5 type A antibody
      • Type 1 cyt b5 antibody
      see all

    References

    This product has been referenced in:
    • Samhan-Arias AK  et al. Stimulation and clustering of cytochrome b(5) reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons. J Proteomics : (2011). WB . Read more (PubMed: 22200675) »
    • Samhan-Arias AK  et al. Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane. Mol Cell Neurosci 40:14-26 (2009). Rat . Read more (PubMed: 18973815) »

    See all 2 Publications for this product

    Publishing research using ab23852? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-Cytochrome b5 antibody

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (cow liver lysate)
    Loading amount
    40 µg
    Specification
    cow liver lysate
    Gel Running Conditions
    Reduced Denaturing (15)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
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    Verified customer

    Submitted Jan 10 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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