Anti-Cytochrome b5 antibody (ab23852)

Product name

Anti-Cytochrome b5 antibody
See all Cytochrome b5 primary antibodies
Description

Goat polyclonal to Cytochrome b5
Host species

Goat
Specificity

Non-specific cross-reactivity with 50 and 60 kDa species.
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Rat, Rabbit, Human
Immunogen

Full length native protein (purified) (Rabbit).

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.05% Sodium azide
Purity

Whole antiserum
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab23852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 15 kDa.

Function

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Involvement in disease

Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
Sequence similarities

Belongs to the cytochrome b5 family.
Contains 1 cytochrome b5 heme-binding domain.
Cellular localization

Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane.
Target information above from: UniProt accession P00167 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1528 Human
- Entrez Gene: 64001 Rat
- Omim: 613218 Human
- SwissProt: P00167 Human
- SwissProt: P00173 Rat
- Unigene: 465413 Human
- Unigene: 1055 Rat
Alternative names
- CYB 5 antibody
- CYB 5A antibody
- CYB5 antibody
- CYB5_HUMAN antibody
- Cyb5a antibody
- Cytochrome b 5 antibody
- Cytochrome b5 (microsomal) antibody
- Cytochrome b5 antibody
- Cytochrome b5 type A (microsomal) antibody
- Cytochrome b5 type A antibody
- MCB 5 antibody
- MCB5 antibody
- Microsomal cytochrome b5 antibody
- Microsomal cytochrome b5 type A antibody
- Type 1 cyt b5 antibody
see all

Samhan-Arias AK et al. Stimulation and clustering of cytochrome b(5) reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons. J Proteomics : (2011). WB . Read more (PubMed: 22200675) »
Samhan-Arias AK et al. Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane. Mol Cell Neurosci 40:14-26 (2009). Rat . Read more (PubMed: 18973815) »

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Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Cow Tissue lysate - whole (cow liver lysate)

Loading amount

40 µg

Specification

cow liver lysate

Gel Running Conditions

Reduced Denaturing (15)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"