• Product name
    Anti-Cytochrome b5 antibody
    See all Cytochrome b5 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome b5
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of Human Cytochrome b5.

  • Positive control
    • NIH/3T3 cells; extracts from A549 cells.



Our Abpromise guarantee covers the use of ab69801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
  • Involvement in disease
    Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
  • Sequence similarities
    Belongs to the cytochrome b5 family.
    Contains 1 cytochrome b5 heme-binding domain.
  • Cellular localization
    Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYB 5 antibody
    • CYB 5A antibody
    • CYB5 antibody
    • CYB5_HUMAN antibody
    • Cyb5a antibody
    • Cytochrome b 5 antibody
    • Cytochrome b5 (microsomal) antibody
    • Cytochrome b5 antibody
    • Cytochrome b5 type A (microsomal) antibody
    • Cytochrome b5 type A antibody
    • MCB 5 antibody
    • MCB5 antibody
    • Microsomal cytochrome b5 antibody
    • Microsomal cytochrome b5 type A antibody
    • Type 1 cyt b5 antibody
    see all


  • All lanes : Anti-Cytochrome b5 antibody (ab69801) at 1/500 dilution

    Lane 1 : extracts from A549 cells
    Lane 2 : extracts from A549 cells with immunizing peptide at 10 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

  • Left: NIH/3T3 cells stained with ab69801 at 1/500 dilution; Right: the antibody was pre-incubated with the immunizing peptide.
  • IHC image of ab69801 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69801, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Reed L  et al. Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice. Arch Toxicol 92:1625-1638 (2018). ELISA ; Mouse . Read more (PubMed: 29368147) »
  • Stark K  et al. CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women. Arthritis Res Ther 17:56 (2015). IHC-P ; Human . Read more (PubMed: 25890314) »

See all 7 Publications for this product

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