Anti-Cytochrome C antibody [37BA11] (ab110325)
- Datasheet
- Specific References (54)
- Protocols
Overview
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Product nameAnti-Cytochrome C antibody [37BA11]
See all Cytochrome C primary antibodies -
DescriptionMouse monoclonal [37BA11] to Cytochrome C
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, In-Cell ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
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Immunogen
Bovine heart Cytochrome C
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Positive control
- Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
PurityIgG fraction
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Purification notesab110325 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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ClonalityMonoclonal
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Clone number37BA11
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IsotypeIgG2a
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Light chain typekappa
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Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab110325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa. | |
| ICC/IF | Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal. | |
| Flow Cyt | Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| In-Cell ELISA | Use a concentration of 4 µg/ml. |
Target
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FunctionElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. -
Involvement in diseaseDefects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
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Sequence similaritiesBelongs to the cytochrome c family.
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Post-translational
modificationsBinds 1 heme group per subunit. -
Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 510767 Cow
- Entrez Gene: 54205 Human
- Entrez Gene: 13063 Mouse
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P62894 Cow
- SwissProt: P99999 Human
- SwissProt: P62897 Mouse
see all -
Alternative names
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)](https://www.abcam.com/ps/products/110/ab110325/Images/ab110325-245075-iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)This image is courtesy of an anonymous Abreviewab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)](https://www.abcam.com/ps/products/110/ab110325/Images/ab110325-4-ab141717smol.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red). -
![Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)](https://www.abcam.com/ps/products/110/ab110325/Images/Cytochrome-C-Primary-antibodies-ab110325-1.jpg)
Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)All lanes : Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Predicted band size: 12 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)](https://www.abcam.com/ps/products/110/ab110325/Images/Cytochrome-C-Primary-antibodies-ab110325-4.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)Immunocytochemistry analysis using ab110325 at 2µg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG. -
![Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)](https://www.abcam.com/ps/products/110/ab110325/Images/Cytochrome-C-Primary-antibodies-ab110325-3.gif)
Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).
Protocols
References
This product has been referenced in:
- Monterisi S et al. PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling. Elife 6:N/A (2017). IHC-P . Read more (PubMed: 28463107) »
- Guo J et al. Inhibition of Fatty Acid Synthase Reduces Blastocyst Hatching through Regulation of the AKT Pathway in Pigs. PLoS One 12:e0170624 (2017). Read more (PubMed: 28107461) »
Customer reviews and Q&As
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