Anti-Cytochrome C antibody [37BA11] (ab110325)

Reviews (4)Q&A (1)References (72)

Overview

  • Product name

    Anti-Cytochrome C antibody [37BA11]
    See all Cytochrome C primary antibodies

  • Description

    Mouse monoclonal [37BA11] to Cytochrome C

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, In-Cell ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans

  • Immunogen

    Bovine heart Cytochrome C

  • Positive control

    • Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
In-Cell ELISA Use a concentration of 4 µg/ml.

Target

  • Function

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

  • Involvement in disease

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

  • Sequence similarities

    Belongs to the cytochrome c family.

  • Post-translational
    modifications

    Binds 1 heme group per subunit.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Images

  • Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
    All lanes : Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 12 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)This image is courtesy of an anonymous Abreview

    ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry analysis using ab110325 at 2µg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG.
  • Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).

References

This product has been referenced in:

  • Li Q  et al. Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice. Int J Biochem Cell Biol 107:154-165 (2019). Read more (PubMed: 30593954) »
  • Zhou XT  et al. Inhibition of autophagy enhances adenosine-induced apoptosis in human hepatoblastoma HepG2 cells. Oncol Rep 41:829-838 (2019). Read more (PubMed: 30535464) »
See all 72 Publications for this product

Publishing research using ab110325? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 24 2018

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Cell lysate - other (Liver, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (12.5 % acrylamide)
Loading amount
60 µg
Specification
Liver, mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 12 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Human Cell (MDA MB 231 cells)
Specification
MDA MB 231 cells
Permeabilization
Yes - 1% Triton
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 19 2015

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Human Cell lysate - other (fractionated cells (nuclear, cytosolic, mitochondr)
Specification
fractionated cells (nuclear, cytosolic, mitochondr
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

Abcam user community

Verified customer

Submitted Nov 28 2014

Question

I want to measure just the cytochrome C secreted from the mitochondria, but not in the mitochondria itself. Is there a lysis buffer or protocol to follow that will do this efficiently?

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Answer

Thank you for your patience in awaiting our reply!

We have 2 products that perfectly fit your experiment:

https://www.abcam.com/Cell-Fractionation-Kit-Standard-ab109719.html

https://www.abcam.com/Cell-Fractionation-Kit-HT-ab109718.html

The products fractionate cells into 3 fractions (cytosolic, mitochondria and nuclear). They were actually validated on cyt c release from mitochondria to cytosol during apoptosis

Also, the fractions generated with these kits are compatible with and were validated on https://www.abcam.com/cytochrome-c-profiling-elisa-kit-ab110172.html https://www.abcam.com/cytochrome-c-profiling-elisa-kit-ab110172.html and https://www.abcam.com/apotrack%E2%84%A2-cytochrome-c-apoptosis-icc-antibody-kit-ab110417.html, in case you also need also a kit to quantify the cyt c.

Please let me know if you have any further questions!

Read More

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