Anti-Cytochrome C antibody [37BA11] (ab110325)

Reviews (4)Q&A (1)References (87)
Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
  • Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)

Key features and details

  • Mouse monoclonal [37BA11] to Cytochrome C
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Cytochrome C antibody [37BA11]
    See all Cytochrome C primary antibodies

  • Description

    Mouse monoclonal [37BA11] to Cytochrome C

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Bovine heart Cytochrome C

  • Positive control

    • Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB (3)
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF (1)
Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
Notes
WB
Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF
Use a concentration of 0.5 - 2 µg/ml. Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

  • Involvement in disease

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

  • Sequence similarities

    Belongs to the cytochrome c family.

  • Post-translational
    modifications

    Binds 1 heme group per subunit.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Images

  • Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)
    All lanes : Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 12 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)This image is courtesy of an anonymous Abreview

    ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Immunocytochemistry analysis using ab110325 at 2µg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG.
  • Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)
    Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).

References (87)

Publishing research using ab110325? Please let us know so that we can cite the reference in this datasheet.

ab110325 has been referenced in 87 publications.

  • Shahid M  et al. Prototheca zopfii genotype II induces mitochondrial apoptosis in models of bovine mastitis. Sci Rep 10:698 (2020). PubMed: 31959834
  • Cohen L  et al. Cardiac remodeling secondary to chronic volume overload is attenuated by a novel MMP9/2 blocking antibody. PLoS One 15:e0231202 (2020). PubMed: 32271823
  • Zhang J  et al. Effects of lysosomal iron involvement in the mechanism of mitochondrial apoptosis on postmortem muscle protein degradation. Food Chem 328:127174 (2020). PubMed: 32492604
  • Yu B  et al. Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics. Nat Commun 11:2549 (2020). PubMed: 32439975
  • Mollica G  et al. L-carnitine supplementation attenuates NAFLD progression and cardiac dysfunction in a mouse model fed with methionine and choline-deficient diet. Dig Liver Dis 52:314-323 (2020). PubMed: 31607566
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 24 2018

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Cell lysate - other (Liver, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (12.5 % acrylamide)
Loading amount
60 µg
Specification
Liver, mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 12 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Human Cell (MDA MB 231 cells)
Specification
MDA MB 231 cells
Permeabilization
Yes - 1% Triton
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 19 2015

Western blot abreview for Anti-Cytochrome C antibody [37BA11]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Human Cell lysate - other (fractionated cells (nuclear, cytosolic, mitochondr)
Specification
fractionated cells (nuclear, cytosolic, mitochondr
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Read More

Abcam user community

Verified customer

Submitted Nov 28 2014

Question

I want to measure just the cytochrome C secreted from the mitochondria, but not in the mitochondria itself. Is there a lysis buffer or protocol to follow that will do this efficiently?

Read More
Answer

Thank you for your patience in awaiting our reply!

We have 2 products that perfectly fit your experiment:

https://www.abcam.com/Cell-Fractionation-Kit-Standard-ab109719.html

https://www.abcam.com/Cell-Fractionation-Kit-HT-ab109718.html

The products fractionate cells into 3 fractions (cytosolic, mitochondria and nuclear). They were actually validated on cyt c release from mitochondria to cytosol during apoptosis

Also, the fractions generated with these kits are compatible with and were validated on https://www.abcam.com/cytochrome-c-profiling-elisa-kit-ab110172.html https://www.abcam.com/cytochrome-c-profiling-elisa-kit-ab110172.html and https://www.abcam.com/apotrack%E2%84%A2-cytochrome-c-apoptosis-icc-antibody-kit-ab110417.html, in case you also need also a kit to quantify the cyt c.

Please let me know if you have any further questions!

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com