Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytochrome C antibody [7H8.2C12]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tubules (kidney))
Specification
Tubules (kidney)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/400
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 594 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500

Additional data

Additional Notes
DAPI was used for staining nucleus

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Submitted Dec 29 2010

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