Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (Heart (mitochondrial and cytosolic fraction))
Loading amount
20 µg
Specification
Heart (mitochondrial and cytosolic fraction)
Gel Running Conditions
Reduced Denaturing (12% Tris glycine SDS PAGE)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 17°C · Diluent: 1% BSA in TBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat polyclonal Secondary Antibody to Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 12 kDa Non-specific: about 65 kDa
Positive control
Mitochondrial cell lysate
Negative control
Nuclear cell lysate

Additional data

Additional Notes
Fantastic antibody. The 12 kDa band is highly specific to the cytochrome C

Abcam user community

Verified customer

Submitted Apr 09 2012

Sign up