Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (Cytosolic fractions from GTN skeletal muscles)
Loading amount
50 µg
Specification
Cytosolic fractions from GTN skeletal muscles
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.05%)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 15 kDa

Additional data

Additional Notes
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors

Dr. G.K Sakellariou

Verified customer

Submitted Sep 28 2012

Sign up