Western blot abreview for Anti-Cytochrome C antibody [7H8.2C12]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS T20

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit anti-mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 15 kDa

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Submitted Jan 07 2013

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