We extracted mitochondrial fraction and cytosolic fraction of the cardiac tissue.
We detected the bands with Western Sure Substrate on a C-Digit Scanner from Li-Cor. Specific bands were seen at about 15kDa. Additional band were detected at about 30kDa. We are not sure if these bands are unspecific or dimeric cytochrome C.
Thank you for your valuable feedback about the use of ab133504 in rabbit, a species we have not tested before. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
Abcam user community
Submitted May 17 2018
Get resources and offers direct to your inboxSign up