The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Predicted molecular weight: 12 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100.
Is unsuitable for Flow Cyt.
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Involvement in disease
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
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Read more (PubMed: 29157834) »
Xu Z et al. The protective effect of propofol against TNF-a-induced apoptosis was mediated via inhibiting iNOS/NO production and maintaining intracellular Ca2+homeostasis in mouse hippocampal HT22 cells. Biomed Pharmacother91:664-672 (2017).
Read more (PubMed: 28499237) »