Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody failed to produce the expected staining pattern under these conditions - rather it produces a strong nuclear-enriched staining pattern. MeOH-fixed samples were also evaluate and did not produce the expected staining pattern.
Thank you for submitting this review. We have followed up with the customer since this should have worked in human cells IF staining. For more details about our Abpromise guarantee please check this link: www.abcam.com/abpromise.
Dr. Kirk Mcmanus
Submitted Apr 04 2014
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