Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome C antibody [EP1326Y]

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody failed to produce the expected staining pattern under these conditions - rather it produces a strong nuclear-enriched staining pattern. MeOH-fixed samples were also evaluate and did not produce the expected staining pattern.
Abcam response
Thank you for submitting this review. We have followed up with the customer since this should have worked in human cells IF staining. For more details about our Abpromise guarantee please check this link: www.abcam.com/abpromise.

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 04 2014

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