Western blot abreview for Anti-Cytochrome C antibody [EP1326-80-5]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (ameloblast derived cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Treatment
fluoride
Specification
ameloblast derived cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Bio-rad anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 12 kDa
Positive control
In the cytosol, Fluoride treatment In the mitochondrial, both fluoride treatment and non-treatment

Additional data

PubMed ID
29185024: View

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Submitted Dec 18 2018

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