Western blot abreview for Anti-Cytochrome C antibody [EP1326-80-5]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SKOV-3)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
SKOV-3
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG HRP-linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 15 kDa

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Submitted Aug 19 2019

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