Product nameCytochrome c Apoptosis ICC Antibody Kit
See all ApoTrack™ Cytochrome c Apoptosis kits
Sample typeAdherent cells
Species reactivityReacts with: Mouse, Rat, Human
Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.
ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.
ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.
This product was previously called ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit.
Other apoptosis assays
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
Tested applicationsSuitable for: ICC/IFmore details
Storage instructionsPlease refer to protocols.
Components Identifier 200 µg 100% normal goat serum 1 x 20ml 10X Buffer (10X PBS, pH 7.4) 1 x 100ml Anti-Complex Va monoclonal antibody clone 15H4C4 1 x 50µg Anti-Cytochrome c monoclonal antibody clone 37BA11 1 x 50µg Detergent (0.1% (v/v) Triton® X-100 in PBS) 1 x 30ml Goat anti-mouse IgG2a - FITC secondary antibody 1 x 50µg Goat anti-mouse IgG2b - TXRD secondary antibody 1 x 50µg
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab110417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
This product has been referenced in:
- Zheng Y et al. Pinocembrin induces ER stress mediated apoptosis and suppresses autophagy in melanoma cells. Cancer Lett 431:31-42 (2018). Read more (PubMed: 29807112) »
- Ismail AM et al. Extra virgin olive oil potentiates the effects of aromatase inhibitors via glutathione depletion in estrogen receptor-positive human breast cancer (MCF-7) cells. Food Chem Toxicol 62:817-24 (2013). Human . Read more (PubMed: 24161488) »