Product nameCytochrome c Apoptosis WB Antibody Cocktail
See all ApoTrack™ Cytochrome c Apoptosis kits
Species reactivityReacts with: Human
The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.
ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse anti GAPDH monclonal:
Working concentration: 0.1 µg/ml
Mouse anti PDH-E1-alpha monoclonal:
Working concentration: 2 µg/ml
Mouse anti C-V-alpha monoclonal:
Working concentration: 0.5 µg/ml
This product was previously called ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail
Other apoptosis assays
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
Tested applicationsSuitable for: WBmore details
Storage instructionsPlease refer to protocols.
Components 180 µg HeLa Lysate Control 1 x 50µg Pre-mixed Solution of 4 monoclonal Antibodies 1 x 180µg
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab110415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting.|
In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
This product has been referenced in:
- Tran LNK et al. The Combination of Metformin and Valproic Acid Induces Synergistic Apoptosis in the Presence of p53 and Androgen Signaling in Prostate Cancer. Mol Cancer Ther 16:2689-2700 (2017). Read more (PubMed: 28802253) »
- Deuse T et al. Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature 509:641-4 (2014). Read more (PubMed: 24747400) »