Cytochrome C Oxidase Assay Kit (ab239711)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Cell Lysate, Tissue, Tissue Lysate
Overview
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Product name
Cytochrome C Oxidase Assay Kit
See all Complex IV kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Tissue, Cell Lysate, Tissue Lysate -
Product overview
Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The kit provides for the fast and simple measurement of Cytochrome c oxidase activity on a 96-well plate format. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K287 Cytochrome Oxidase Activity Colorimetric Assay Kit. K287-100 is the same size as the 100 test size of ab239711.
Range of complex IV / cytochrome c oxidase assay kits
Biochemical assay - ab239711 (this kit)
Immunocapture with biochemical assay (plate-based)*** - ab109911 (rodent) and ab109909 (human)
*** Most popular assay formatImmunocapture with biochemical assay (dipstick) - ab109878 (rodent) and ab109876 (human)
Immunocapture with biochemical assay and ELISA - ab109910 (human)
ELISA - ab179880 (human)
Other related productsReview the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 1 M DTT 1 x 1ml 96-well Plate 1 unit Cytochrome c 2 vials Cytochrome Oxidase Assay Buffer 1 x 25ml Enzyme Dilution Buffer 1 x 8ml -
Research areas
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Alternative names
- cco
- COX
- Cytochrome c oxidase
- Cytochrome oxidase
Images
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Cytochrome Oxidase Activity
Purified mitochondria were used to calculate a decrease in OD at 550 nm (0.625-5 µg/reaction). In Blank, no change in the OD was observed. Rate is calculated by subtracting the initial OD reading from the final OD, t1 and t2 represents linear rate of reaction.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab239711 has been referenced in 3 publications.
- Cui L et al. Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. Nat Biotechnol 39:357-367 (2021). PubMed: 33077961
- Seira O et al. Ketogenesis controls mitochondrial gene expression and rescues mitochondrial bioenergetics after cervical spinal cord injury in rats. Sci Rep 11:16359 (2021). PubMed: 34381166
- Hu K et al. Marriage of black phosphorus and Cu2+ as effective photothermal agents for PET-guided combination cancer therapy. Nat Commun 11:2778 (2020). PubMed: 32513979