Cytochrome C Oxidase Assay Kit (ab239711)

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Cytochrome Oxidase Activity

    Key features and details

    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: Cell culture supernatant, Cell Lysate, Tissue, Tissue Lysate

    Overview

    • Product name

      Cytochrome C Oxidase Assay Kit
      See all Complex IV kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Tissue, Cell Lysate, Tissue Lysate

    • Product overview

      Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The kit provides for the fast and simple measurement of Cytochrome c oxidase activity on a 96-well plate format. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.

    • Notes

      Range of complex IV / cytochrome c oxidase assay kits

      Biochemical assay - ab239711 (this kit)

      Immunocapture with biochemical assay (plate-based)*** - ab109911 (rodent)  and ab109909 (human)
      *** Most popular assay format

      Immunocapture with biochemical assay (dipstick) - ab109878 (rodent) and ab109876 (human)

      Immunocapture with biochemical assay and ELISA - ab109910 (human) 

      ELISA - ab179880 (human)


      Other related products

      Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

    • Platform

      Microplate reader

    Properties

    Images

    • Cytochrome Oxidase Activity
      Cytochrome Oxidase Activity

      Purified mitochondria  were used to calculate a decrease in OD at 550 nm (0.625-5 µg/reaction). In Blank, no change in the OD was observed. Rate is calculated by subtracting the initial OD reading from the final OD, t1 and t2 represents linear rate of reaction.

       

       

    References (2)

    Publishing research using ab239711? Please let us know so that we can cite the reference in this datasheet.

    ab239711 has been referenced in 2 publications.

    • Cui L  et al. Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. Nat Biotechnol 39:357-367 (2021). PubMed: 33077961
    • Hu K  et al. Marriage of black phosphorus and Cu2+ as effective photothermal agents for PET-guided combination cancer therapy. Nat Commun 11:2778 (2020). PubMed: 32513979

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