Overview

  • Product name

    Cytochrome C Oxidase Assay Kit
    See all Complex IV kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Tissue, Cell Lysate, Tissue Lysate
  • Product overview

    Cytochrome C Oxidase Activity Assay Kit (ab239711) is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The kit provides for the fast and simple measurement of Cytochrome c oxidase activity on a 96-well plate format. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.

  • Notes

    Range of complex IV / cytochrome c oxidase assay kits

    Biochemical assay - ab239711 (this kit)

    Immunocapture with biochemical assay (plate-based)*** - ab109911 (rodent)  and ab109909 (human)
    *** Most popular assay format

    Immunocapture with biochemical assay (dipstick) - ab109878 (rodent) and ab109876 (human)

    Immunocapture with biochemical assay and ELISA - ab109910 (human) 

    ELISA - ab179880 (human)


    Other related products

    Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

  • Platform

    Microplate reader

Properties

Images

  • Purified mitochondria  were used to calculate a decrease in OD at 550 nm (0.625-5 µg/reaction). In Blank, no change in the OD was observed. Rate is calculated by subtracting the initial OD reading from the final OD, t1 and t2 represents linear rate of reaction.

     

     

Protocols

References

ab239711 has not yet been referenced specifically in any publications.

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