Recombinant Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cytochrome P450 17A1/CYP17A1 aa 100-200. The exact sequence is proprietary.
Database link: P05093 -
Positive control
- WB: Human adrenal gland, mouse and rat heart lysates, human fetal heart lysate, SK-OV-3, HeLa and Jurkat lysates; Flow Cyt (intra): HeLa cells ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6293 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203743)
- Alexa Fluor® 488 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203995)
- HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)
- PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab223958)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - Low endotoxin, Azide free (ab226009)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - BSA and Azide free (ab271888)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
|
IP |
1/10 - 1/100.
|
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ICC/IF | (1) |
1/100 - 1/250.
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa). |
IP
1/10 - 1/100. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. -
Pathway
Lipid metabolism; steroid biosynthesis. -
Involvement in disease
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic). -
Sequence similarities
Belongs to the cytochrome P450 family. -
Post-translational
modificationsPhosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1586 Human
- Omim: 609300 Human
- SwissProt: P05093 Human
- Unigene: 438016 Human
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Alternative names
- 20 lyase antibody
- CP17A_HUMAN antibody
- CPT7 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg
Predicted band size: 57 kDa -
ab125022 (purified) at 1:30 dilution (2µg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
Lane 1 (input): Human fetal heart lysate 10µg
Lane 2 (+): ab125022 & Human fetal heart lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125022 in Human fetal heart lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution
Lane 1 : Human adrenal gland lysate
Lane 2 : SK-OV-3 lysate
Lane 3 : HeLa lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab125022 has been referenced in 31 publications.
- Zhang Y et al. Zika Virus Infection in the Ovary Induces a Continuously Elevated Progesterone Level and Compromises Conception in Interferon Alpha/Beta Receptor-Deficient Mice. J Virol 96:e0118921 (2022). PubMed: 34730391
- Gudelska M et al. Chemerin Affects P4 and E2 Synthesis in the Porcine Endometrium during Early Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 35055130
- Luo Q et al. hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms. Reprod Biol Endocrinol 20:125 (2022). PubMed: 35986315
- Piprek RP et al. Desmoplakin (Dsp) conditional knockout in NR5A1+ somatic cells affects germ cell survival in developing mouse gonads. Reproduction 163:199-207 (2022). IHC ; Mouse . PubMed: 35119382
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531