Overview

  • Product name

    Anti-Cytochrome P450 17A1/CYP17A1 antibody
    See all Cytochrome P450 17A1/CYP17A1 primary antibodies
  • Description

    Rabbit polyclonal to Cytochrome P450 17A1/CYP17A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cytochrome P450 17A1/CYP17A1 (internal sequence) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • K562 cell line lysates
  • General notes

     This product was previously labelled as Cytochrome P450 17A1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab80206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
  • Pathway

    Lipid metabolism; steroid biosynthesis.
  • Involvement in disease

    Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Post-translational
    modifications

    Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 20 lyase antibody
    • CP17A_HUMAN antibody
    • CPT7 antibody
    • CYP17 antibody
    • CYP17A1 antibody
    • CYPXVII antibody
    • Cytochrome P450 17A1 antibody
    • Cytochrome P450 family 17 antibody
    • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
    • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
    • Cytochrome p450 XVIIA1 antibody
    • Cytochrome P450-C17 antibody
    • Cytochrome P450c17 antibody
    • OTTHUMP00000020382 antibody
    • P450 C17 antibody
    • P450c17 antibody
    • S17AH antibody
    • Steroid 17 alpha hydroxylase/17,20 lyase antibody
    • Steroid 17 alpha monooxygenase antibody
    • Steroid 17-alpha-hydroxylase/17 antibody
    • Steroid 17-alpha-monooxygenase antibody
    see all

Images

  • Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab80206) at 1/50 dilution + K562 cell line lysates at 35 µg

    Predicted band size: 57 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab80206 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80206, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab80206 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80206, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Rodríguez-Sanz M  et al. CYP11A1 expression in bone is associated with aromatase inhibitor-related bone loss. J Mol Endocrinol 55:69-79 (2015). Read more (PubMed: 26108486) »
  • Fujii H  et al. Marked cortisol production by intracrine ACTH in GIP-treated cultured adrenal cells in which the GIP receptor was exogenously introduced. PLoS One 9:e110543 (2014). Read more (PubMed: 25334044) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ovary)
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Ms. Natasja Costermans

Verified customer

Submitted Nov 09 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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