Recombinant
RabMAb

Recombinant Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Overview

  • Product name

    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
    See all Cytochrome P450 17A1/CYP17A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cytochrome P450 17A1/CYP17A1 aa 100-200. The exact sequence is proprietary.
    Database link: P05093

  • Positive control

    • WB: Human adrenal gland, mouse and rat heart lysates, human fetal heart lysate, SK-OV-3, HeLa and Jurkat lysates; FC: HeLa cells ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as Cytochrome P450 17A1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab125022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
    • Pathway

      Lipid metabolism; steroid biosynthesis.
    • Involvement in disease

      Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Post-translational
      modifications

      Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 20 lyase antibody
      • CP17A_HUMAN antibody
      • CPT7 antibody
      • CYP17 antibody
      • CYP17A1 antibody
      • CYPXVII antibody
      • Cytochrome P450 17A1 antibody
      • Cytochrome P450 family 17 antibody
      • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
      • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
      • Cytochrome p450 XVIIA1 antibody
      • Cytochrome P450-C17 antibody
      • Cytochrome P450c17 antibody
      • OTTHUMP00000020382 antibody
      • P450 C17 antibody
      • P450c17 antibody
      • S17AH antibody
      • Steroid 17 alpha hydroxylase/17,20 lyase antibody
      • Steroid 17 alpha monooxygenase antibody
      • Steroid 17-alpha-hydroxylase/17 antibody
      • Steroid 17-alpha-monooxygenase antibody
      see all

    Images

    • All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (Purified)

      Lane 1 : Mouse heart lysates
      Lane 2 : Rat heart lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg

      Predicted band size: 57 kDa

    • ab125022 (purified) at 1:30 dilution (2µg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
      Lane 1 (input): Human fetal heart lysate 10µg
      Lane 2 (+): ab125022 & Human fetal heart lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125022 in Human fetal heart lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

      Lane 1 : Human adrenal gland lysate
      Lane 2 : SK-OV-3 lysate
      Lane 3 : HeLa lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    References

    This product has been referenced in:

    • Choi SR  et al. Spinal cytochrome P450c17 plays a key role in the development of neuropathic mechanical allodynia: Involvement of astrocyte sigma-1 receptors. Neuropharmacology 149:169-180 (2019). Read more (PubMed: 30797030) »
    • Prasad SK  et al. Radioprotective effect of ethanolic extract of Alocasia indica on ?-irradiation-induced reproductive alterations in ovary and uterus. Int J Radiat Biol N/A:1-14 (2019). Read more (PubMed: 31314632) »
    See all 11 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution low pH
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Prostate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formalin

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Jun 25 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Prostate Cancer Cell Lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Treatment
    Hormone treatment
    Specification
    Prostate Cancer Cell Lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Ms. Virginia Fasoulaki

    Verified customer

    Submitted Sep 27 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (H1299 Lung cancer cell line)
    Permeabilization
    Yes - 0.1% v/v TritonX-100
    Specification
    H1299 Lung cancer cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Dr. Dimitra Kalamida

    Verified customer

    Submitted May 31 2016

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