Anti-CYP1A1 antibody (ab235185)
Key features and details
- Rabbit polyclonal to CYP1A1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP1A1 antibody
See all CYP1A1 primary antibodies -
Description
Rabbit polyclonal to CYP1A1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: HepG2 cells. IHC-P: Human kidney tissue. WB: CYP1A1 overexpressing lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 15 µg/ml.
Incubate with primary antibody overnight at 4°C. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT. |
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WB |
1/1000. Predicted molecular weight: 58 kDa.
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Notes |
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ICC/IF
Use a concentration of 15 µg/ml. Incubate with primary antibody overnight at 4°C. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at RT. |
WB
1/1000. Predicted molecular weight: 58 kDa. |
Target
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Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
Tissue specificity
Lung, lymphocytes and placenta. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1543 Human
- Omim: 108330 Human
- SwissProt: P04798 Human
- Unigene: 72912 Human
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Alternative names
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
see all
Images
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All lanes : Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution
Lane 1 : CYP1A1 overexpressing lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) lysate
Secondary
All lanes : Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution
Predicted band size: 58 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.
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Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.
Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.
Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.
A. Human kidney tissue counterstained with Hematoxilyn-Eosin.
B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).
C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 µg/ml in ICC/IF.
Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 µg/ml for 1 hour at room temperature.
Panel A: DAPI (blue)
Panel B: ab235185 + secondary antibody (green)
Panel C: Actin-Phalloidin 555 (red)
Panel D: Secondary antibody only
Panel E: Merge A+B
Panel F: Merge A+B+C
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab235185 has been referenced in 1 publication.
- Groestlinger J et al. Combinatory Exposure to Urolithin A, Alternariol, and Deoxynivalenol Affects Colon Cancer Metabolism and Epithelial Barrier Integrity in vitro. Front Nutr 9:882222 (2022). PubMed: 35811943