Recombinant
RabMAb

Recombinant Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

Overview

  • Product name

    Anti-Cytochrome P450 1A2 antibody [EPR6138(2)]
    See all Cytochrome P450 1A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6138(2)] to Cytochrome P450 1A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytochrome P450 1A2 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: Caco2, HepG2, HeLa and A549 cell lysates. ICC/IF: HeLa cells. FC: MCF7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
Flow Cyt 1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/200 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
    • Tissue specificity

      Liver.
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aryl hydrocarbon hydroxylase antibody
      • CP 12 antibody
      • CP12 antibody
      • CP1A2_HUMAN antibody
      • CYP1A2 antibody
      • CYPIA2 antibody
      • Cytochrome P(3)450 antibody
      • Cytochrome P450 1A2 antibody
      • Cytochrome P450 4 antibody
      • Cytochrome P450 family 1 polypeptide 2 antibody
      • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
      • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
      • Cytochrome P450-P3 antibody
      • Cytochrome P4501A2 antibody
      • Dioxin inducable P3 450 antibody
      • Flavoprotein linked monooxygenase antibody
      • Flavoprotein-linked monooxygenase antibody
      • Microsomal monooxygenase antibody
      • P(3)450 antibody
      • P3 450 antibody
      • P450 4 antibody
      • P450 form 4 antibody
      • P450 P3 antibody
      • P450(PA) antibody
      • Xenobiotic monooxygenase antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (9.4 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728) at 1/1000 dilution ((unpurified))

      Lane 1 : Caco2 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunofluorescent analysis of HeLa cells labeling Cytochrome P450 1A2 with unpurified ab151728 at 1/250 dilution.

    • Overlay histogram showing MCF7 cells stained with unpurified ab151728 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151728, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    This product has been referenced in:

    • Gill P  et al. MicroRNA regulation of CYP 1A2, CYP3A4 and CYP2E1 expression in acetaminophen toxicity. Sci Rep 7:12331 (2017). WB . Read more (PubMed: 28951593) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    Unfortunately, I am sorry to confirm we did not specifically test ab151728 CYP1A antibody for this cross reactivity. However, we did a BLAST with the immunogen sequence and this indicates that theoretically this antibody will not cross react with other CYP1A proteins. We ran an alignment, specifically with the sequence for CYP1A1, and the homology is very low (not significant).

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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