Overview

  • Product name
    Anti-Cytochrome P450 2A6 antibody
    See all Cytochrome P450 2A6 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2A6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type (His-tag) corresponding to Human Cytochrome P450 2A6. Purified, His-tagged, full length human P450 2A6 fusion protein.

  • Positive control
    • Rat liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa).

This antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract.

Target

  • Function
    Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Possesses low phenacetin O-deethylation activity.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coumarin 7 hydroxylase antibody
    • Coumarin 7-hydroxylase antibody
    • CP2A6_HUMAN antibody
    • CPA6 antibody
    • CYP2A antibody
    • CYP2A3 antibody
    • CYP2A6 antibody
    • CYPIIA6 antibody
    • Cytochrome P450 2A6 antibody
    • Cytochrome P450 IIA3 antibody
    • Cytochrome P450 subfamily IIA (phenobarbital inducible) polypeptide 6 antibody
    • Cytochrome P450(I) antibody
    • Cytochrome P450, family 2 subfamily A polypeptide 6 antibody
    • Flavoprotein linked monooxygenase antibody
    • P450C2A antibody
    • P450PB antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • ab3570 at a dilution of 1/2000 staining cytochrome P450 2A6 in rat liver lysate by Western blot.

References

ab3570 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffet, pH6
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 06 2017

Answer

Thank you for your response. While I cannot retroactively apply a discount to the order you have placed, the code will entitle you to xx off of one primary antibody for a future purchase. The code must be used on or before 10 October 2012. I would like to encourage you to take advantage of our Abreview program. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. To leave and Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and clickSubmit an Abreview. You can gain more Abpoints by submitting images as well. I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Question

Hello,

I am a graduate student at University of the Sciences in Philadelphia, I recently ordered and received the ab53027 Anti-CYP2A6 antibody.
I have been using it to try to detect CYP2A6 expression in both Human Liver Microsomes and microsomes I isolated from cell culture.
I have been using it at a 1:1000 dilution - 10uL in 10mL of my blocking buffer, thus far I have had no success detecting the cell's microsomes. I have been able to consistently detect the liver microsomes so I know that my blotting procedure works, the antibody is functional and that I have an appropriate secondary antibody and chemiluminescent stain.
On the product information insert it says to use the antibody at a 1/150 dilution for western blots, this seems quite high. Is it that the antibody is has weak affinity for CYP2A6 and needs to be at such a high concentration?
Also, when I attempted to visit the abcam.com website for more information I found that this particular antibody is no longer available. Is this just a supply issue, or is there a functional reason for this change?
Please advise on what volume of antibody you recommend for me to incubate with my blot, and in what volume of blocking buffer, as well as the duration of incubation i.e. 1 hour, 2 hours, overnight etc.

Thank you for your time.

Please let me know if you need any more clarification on my procedure.

Stephanie L. Schaffer
BS in Pharmacology/Toxicology 2009
MS in Pharmacology/Toxicology 2012
University of the Sciences in Philadelphia
mailto:sschaffer@mail.usip.edu
http://www.usip.edu/sga

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Answer

Thank you for contacting us. The recommended dilution of 1:150 is a recommendation which we make to our customers based on our experiments or those of supplier, collaborator or customer input. Although we cannot know exactly why certain products require higher dilutions in different protocols issues of affinity or reaction conditions will play a role. I would recommend using this product at the higher concentration recommended on the datasheet. I would also recommend running a positive control at the same time using the human liver Microsomes and strongly recommend using a loading control such as Beta Actin or GAPDH.They are used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes. I have included links to these loading controls below. As you have not yet seen expression in your sample cell line I would also recommend a longer incubation, 4C overnight. We currently have a catalog of over 70,000 products. While we do make many of our products in house, we do receive others from outside suppliers. This product has been discontinued by our supplier; we do not know the exact cause for this discontinuation. We do offer a number of other CytochromeP450 2A6 antibodies which we do guarantee to work in western blot on cell lines. Our Rabbit polyclonal antibodies to Cytochrome P450 2A6, AB64890 or AB3570 may best suit your needs. As this product was discontinued shortly after your purchase I can offer you a discount off of anotherCytochrome P450 2A6 antibody should you decide to try one. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Beta Actin Loading Control:https://www.abcam.com/beta-Actin-antibody-mAbcam-8226-Loading-Control-ab8226.html GAPDH Loading Control:https://www.abcam.com/GAPDH-antibody-mAbcam-9484-Loading-Control-ab9484.html Further information about use of loading controls may be found at the following link:https://www.abcam.com/index.html?pageconfig=resource&rid=275 Two of our otherCytochrome P450 2A6 antibodies: AB3570:https://www.abcam.com/Cytochrome-P450-2A6-antibody-ab3570.html AB64890:https://www.abcam.com/Cytochrome-P450-2A6-antibody-ab64890.html

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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