Overview

  • Product name
    Anti-Cytochrome P450 2A6 antibody
    See all Cytochrome P450 2A6 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2A6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type (His-tag) corresponding to Human Cytochrome P450 2A6. Purified, His-tagged, full length human P450 2A6 fusion protein.

  • Positive control
    • Rat liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa).

This antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract.

Target

  • Function
    Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Possesses low phenacetin O-deethylation activity.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coumarin 7 hydroxylase antibody
    • Coumarin 7-hydroxylase antibody
    • CP2A6_HUMAN antibody
    • CPA6 antibody
    • CYP2A antibody
    • CYP2A3 antibody
    • CYP2A6 antibody
    • CYPIIA6 antibody
    • Cytochrome P450 2A6 antibody
    • Cytochrome P450 IIA3 antibody
    • Cytochrome P450 subfamily IIA (phenobarbital inducible) polypeptide 6 antibody
    • Cytochrome P450(I) antibody
    • Cytochrome P450, family 2 subfamily A polypeptide 6 antibody
    • Flavoprotein linked monooxygenase antibody
    • P450C2A antibody
    • P450PB antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • ab3570 at a dilution of 1/2000 staining cytochrome P450 2A6 in rat liver lysate by Western blot.

References

ab3570 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffet, pH6
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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