Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)] (ab22721)
Key features and details
- Mouse monoclonal [b/e4 (9.20)] to Cytochrome P450 2B1 + 2B2
- Suitable for: ELISA, WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)]
See all Cytochrome P450 2B1 + 2B2 primary antibodies -
Description
Mouse monoclonal [b/e4 (9.20)] to Cytochrome P450 2B1 + 2B2 -
Host species
Mouse -
Specificity
Clone b/e4 does not react with CYP 1A1, 1A2, 2C6, 2C7, 2C11, 4A2, 4A3. -
Tested applications
Suitable for: ELISA, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Full length protein (Rat).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
b/e4 (9.20) -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Relevance
The Cytochrome P450 superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. -
Cellular localization
Membrane bound, endoplasmic reticulum -
Database links
- Entrez Gene: 24300 Rat
- Entrez Gene: 361523 Rat
- SwissProt: P00176 Rat
- SwissProt: P04167 Rat
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Alternative names
- Cyp2b 1 antibody
- Cyp2b 2 antibody
- Cyp2b1 antibody
see all
Datasheets and documents
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Datasheet download
References (1)
ab22721 has been referenced in 1 publication.
- Probert PM et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). WB ; Rat . PubMed: 24235770