Product nameAnti-Cytochrome P450 2B6/CYP2B6 antibody
See all Cytochrome P450 2B6/CYP2B6 primary antibodies
DescriptionGoat polyclonal to Cytochrome P450 2B6/CYP2B6
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- WB: HepG2 and K562 cell lysates.
This product was previously labelled as Cytochrome P450 2B6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223511 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab223511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 56.3 kDa).|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Tissue specificityExpressed in liver, lung and heart right ventricle.
Sequence similaritiesBelongs to the cytochrome P450 family.
modificationsPhosphorylation is accompanied by a decrease in enzyme activity.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- 1,4 cineole 2 exo monooxygenase antibody
- 11B1 antibody
- CP2B6_HUMAN antibody
All lanes : Anti-Cytochrome P450 2B6/CYP2B6 antibody (ab223511) at 0.1 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate in RIPA buffer
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 56.3 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223511 has not yet been referenced specifically in any publications.