Overview

  • Product name

    Anti-Cytochrome P450 2B6/CYP2B6 antibody
    See all Cytochrome P450 2B6/CYP2B6 primary antibodies
  • Description

    Goat polyclonal to Cytochrome P450 2B6/CYP2B6
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Cytochrome P450 2B6/CYP2B6 aa 247-259 (internal sequence) (Cysteine residue). NP_000758.1.
    Sequence:

    C-HSVEKHRETLDPS


    Database link: P20813

  • Positive control

    • WB: HepG2 and K562 cell lysates.
  • General notes

     This product was previously labelled as Cytochrome P450 2B6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab223511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 56.3 kDa).

Target

  • Function

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificity

    Expressed in liver, lung and heart right ventricle.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Post-translational
    modifications

    Phosphorylation is accompanied by a decrease in enzyme activity.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1,4 cineole 2 exo monooxygenase antibody
    • 11B1 antibody
    • CP2B6_HUMAN antibody
    • CPB 6 antibody
    • CPB6 antibody
    • CYP 2B6 antibody
    • CYP IIB6 antibody
    • CYP2B antibody
    • CYP2B6 antibody
    • CYP2B7 antibody
    • CYP2B7P antibody
    • CYPIIB6 antibody
    • Cytochrome P450 2B6 antibody
    • Cytochrome P450 family 2 subfamily B antibody
    • Cytochrome P450 family 2 subfamily B polypeptide 6 antibody
    • Cytochrome P450 IIB1 antibody
    • Cytochrome P450 phenobarbital inducible antibody
    • Cytochrome P450 subfamily IIB (phenobarbital inducible) antibody
    • Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6 antibody
    • Cytochrome P450 subfamily IIB polypeptide 6 antibody
    • EFVM antibody
    • IIB1 antibody
    • P450 antibody
    • P450 IIB1 antibody
    • P450IIB1 antibody
    see all

Images

  • All lanes : Anti-Cytochrome P450 2B6/CYP2B6 antibody (ab223511) at 0.1 µg/ml

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate in RIPA buffer
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56.3 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223511 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse myoblasts)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
Mouse myoblasts
Blocking step
Fish gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Jildou van der Werf

Verified customer

Submitted May 09 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up