Recombinant
RabMAb

Recombinant Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609)

Overview

  • Product name

    Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)]
    See all Cytochrome P450 2B6/CYP2B6 primary antibodies
  • Description

    Rabbit monoclonal [EPR9985(B)] to Cytochrome P450 2B6/CYP2B6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cytochrome P450 2B6/CYP2B6 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • HepG2 whole cell lysate (ab7900) and fetal kidney lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Cytochrome P450 2B6

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    • Tissue specificity

      Expressed in liver, lung and heart right ventricle.
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Post-translational
      modifications

      Phosphorylation is accompanied by a decrease in enzyme activity.
    • Cellular localization

      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1,4 cineole 2 exo monooxygenase antibody
      • 11B1 antibody
      • CP2B6_HUMAN antibody
      • CPB 6 antibody
      • CPB6 antibody
      • CYP 2B6 antibody
      • CYP IIB6 antibody
      • CYP2B antibody
      • CYP2B6 antibody
      • CYP2B7 antibody
      • CYP2B7P antibody
      • CYPIIB6 antibody
      • Cytochrome P450 2B6 antibody
      • Cytochrome P450 family 2 subfamily B antibody
      • Cytochrome P450 family 2 subfamily B polypeptide 6 antibody
      • Cytochrome P450 IIB1 antibody
      • Cytochrome P450 phenobarbital inducible antibody
      • Cytochrome P450 subfamily IIB (phenobarbital inducible) antibody
      • Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6 antibody
      • Cytochrome P450 subfamily IIB polypeptide 6 antibody
      • EFVM antibody
      • IIB1 antibody
      • P450 antibody
      • P450 IIB1 antibody
      • P450IIB1 antibody
      see all

    Images

    • All lanes : Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 56 kDa

    References

    This product has been referenced in:

    • Valencia-Cervantes J  et al. Hypoxia increases chemoresistance in human medulloblastoma DAOY cells via hypoxia-inducible factor 1a-mediated downregulation of the CYP2B6, CYP3A4 and CYP3A5 enzymes and inhibition of cell proliferation. Oncol Rep 41:178-190 (2019). Read more (PubMed: 30320358) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK17 cell line)
    Gel Running Conditions
    Reduced Denaturing (SDS-PAGE Gel 10%)
    Loading amount
    40 µg
    Specification
    BHK17 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 05 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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