Recombinant Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9985(B)] to Cytochrome P450 2B6/CYP2B6
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)]
See all Cytochrome P450 2B6/CYP2B6 primary antibodies -
Description
Rabbit monoclonal [EPR9985(B)] to Cytochrome P450 2B6/CYP2B6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cytochrome P450 2B6/CYP2B6 aa 300-400. The exact sequence is proprietary.
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Positive control
- HepG2 whole cell lysate (ab7900) and fetal kidney lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9985(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
Tissue specificity
Expressed in liver, lung and heart right ventricle. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Post-translational
modificationsPhosphorylation is accompanied by a decrease in enzyme activity. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1555 Human
- Omim: 123930 Human
- SwissProt: P20813 Human
- Unigene: 1360 Human
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Alternative names
- 1,4 cineole 2 exo monooxygenase antibody
- 11B1 antibody
- CP2B6_HUMAN antibody
see all
Images
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All lanes : Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell from embryonic kidney transformed with large T antigen) transfected with an empty expression vector, whole cell lysate
Lane 2 : HEK-293T transfected with full length His-tagged Human CYP2B6 expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 56 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab140609 has been referenced in 1 publication.
- Valencia-Cervantes J et al. Hypoxia increases chemoresistance in human medulloblastoma DAOY cells via hypoxia-inducible factor 1a-mediated downregulation of the CYP2B6, CYP3A4 and CYP3A5 enzymes and inhibition of cell proliferation. Oncol Rep 41:178-190 (2019). PubMed: 30320358