Recombinant
RabMAb

Recombinant Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Overview

  • Product name

    Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal
    See all Cytochrome p450 2C19 primary antibodies
  • Description

    Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P33261

  • Positive control

    • HeLa cells, 293 cells, Human fetal liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
Flow Cyt 1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40 - 1/60.
ICC/IF 1/250.

Target

  • Function

    Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • (R)-limonene 6-monooxygenase antibody
    • (S)-limonene 6-monooxygenase antibody
    • (S)-limonene 7-monooxygenase antibody
    • CP2CJ_HUMAN antibody
    • CPCJ antibody
    • CYP2C antibody
    • CYP2C19 antibody
    • CYPIIC17 antibody
    • CYPIIC19 antibody
    • Cytochrome P-450 II C antibody
    • Cytochrome P450 2C19 antibody
    • Cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19 antibody
    • Cytochrome P450-11A antibody
    • Cytochrome P450-254C antibody
    • Flavoprotein-linked monooxygenase antibody
    • Mephenytoin 4 hydroxylase antibody
    • Mephenytoin 4-hydroxylase antibody
    • Microsomal monooxygenase antibody
    • P450-11A antibody
    • P45011A antibody
    • P450C2C antibody
    • S-mephenytoin 4-hydroxylase antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Cytochrome P450 2C19 with ab185213 at 1/240 (red) or Rabbit monoclonal IgG (green) followed by Goat anti rabbit IgG (FITC) at 1/150.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling Cytochrome P450 2C19 with ab185213 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 and counterstained with Dapi.

  • Immunoprecipitation analysis of 293 cell lysate immunoprecipitated for Cytochrome P450 2C19 using ab185213 at 1/50 dilution (Lane 1). A aAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution was used as secondary. Lane 2: PBS instead of 293 lysates

  • All lanes : Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/5000 dilution

    Lane 1 : HeLa cell line lysate
    Lane 2 : 293 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

References

ab185213 has not yet been referenced specifically in any publications.

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