Overview

  • Product name
    Anti-Cytochrome P450 2C9 antibody
    See all Cytochrome P450 2C9 primary antibodies
  • Description
    Rabbit polyclonal to Cytochrome P450 2C9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human) expressed in E. coli.

  • General notes


    The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Properties

Applications

Our Abpromise guarantee covers the use of ab4236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 59 kDa. Positive control 0.1 µg of recombinant human cytochrome P450 2

Target

  • Function
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • (R)-limonene 6-monooxygenase antibody
    • (S)-limonene 6-monooxygenase antibody
    • (S)-limonene 7-monooxygenase antibody
    • CP2C9_HUMAN antibody
    • CPC9 antibody
    • CYP2C antibody
    • CYP2C10 antibody
    • CYP2C9 antibody
    • CYPIIC9 antibody
    • cytochrome P-450 S-mephenytoin 4-hydroxylase antibody
    • Cytochrome P-450MP antibody
    • Cytochrome P450 2C9 antibody
    • Cytochrome P450 MP-4 antibody
    • Cytochrome P450 MP-8 antibody
    • Cytochrome P450 PB-1 antibody
    • Cytochrome P450, family 2, subfamily C, polypeptide 9 antibody
    • Cytochrome p4502C9 antibody
    • flavoprotein-linked monooxygenase antibody
    • MGC149605 antibody
    • MGC88320 antibody
    • microsomal monooxygenase antibody
    • OTTHUMP00000020135 antibody
    • P450 MP antibody
    • P450 PB 1 antibody
    • P450 PB1 antibody
    • P450IIC9 antibody
    • P450MP antibody
    • S mephenytoin 4 hydroxylase antibody
    • S-mephenytoin 4-hydroxylase antibody
    • xenobiotic monooxygenase antibody
    see all

Images

  • IHC image of ab4236 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4236, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Guo R  et al. Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor. Stem Cell Reports 9:499-512 (2017). Read more (PubMed: 28757167) »
  • Skrypnyk N  et al. PPARa activation can help prevent and treat non-small cell lung cancer. Cancer Res 74:621-31 (2014). Read more (PubMed: 24302581) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your reply and for sending your data. I agree that the results you have obtained are difficult to interpret. For that reason, I would be happy to offer you free of charge replacements, or a credit or refund. Please let me know how you would like to proceed and I will be happy to assist you further.

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Question
Answer

Thank you for contacting us.
Because ab4236 is a rabbit polyclonal antibody that was raised against a full length protein, you could use a full length recombinant Cytochrome P450 2C9 protein such as ab56998 for blocking. It is very difficult and expensive to achieve full blocking using a full length protein, however you may be able to observe a reduction in the specific signal by pre-incubation with this product. After checking with the lab, a blocking peptide for ab110746 is not currently available.
I am sorry I could not be more helpful, please let me know if you need any additional information or assistance.

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Question
Answer

DISCOUNT CODE: ***
Expiration date: May 26th, 2012

I am very pleased to hear you would like to accept our offer and test ab4236 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver)
Loading amount
80 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Joseph Statwick

Verified customer

Submitted Mar 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 13 2010

Question
Answer

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and endothelial cells but not whole skin in the following paper: Leukotriene A4 hydrolase in human skin. J Invest Dermatol. 1994 Feb;102(2):253-7. PMID: 8106755 Our lysate ab30166 may be suitable as a negative control but I cannot guarantee you will not see some reactivity. For the cytochrome P450 enzymes, I suggest liver lysates. A positive control for ab4236 is human liver: ab29889. For ab13513 and ab3573, rat liver lysate ab29413 will be appropriate. I am reluctant to recommend a negative control since information regarding absence of expression is difficult to come by but since CP450 is associated with the ER, cytoplasmic fractions of liver lysates (which do not contain membrane) may be useful. For human liver, this would be ab29875, and for rat liver ab29409. You should also have a look at the references listed on the datasheets to see how other researchers have used the antibodies, though the references for the LTA4 are general and not specific to the antibody. I hope this helps. Please do not hesitate to contact us if you have any other questions.

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