Overview

  • Product name
    Anti-Cytochrome P450 2D4 antibody
  • Description
    Rabbit polyclonal to Cytochrome P450 2D4
  • Host species
    Rabbit
  • Specificity
    ab22592 recognises Cytochrome P450 2D4
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic pentapeptide which corresponding to the C terminal of rat liver Cytochrome P450 2D4 which was conjugated to keyhole limpet haemocyanin using a maleimido linkage.

  • Positive control
    • clozapine treated rat brain preparations
  • General notes
    ab22592 is not suitable for human studies.

Properties

Applications

Our Abpromise guarantee covers the use of ab22592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.
IHC-Fr 1/1000. Using ABC Elite detection.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Cellular localization
    Endoplasmic reticulum
  • Database links
  • Alternative names
    • Cyp2d18 antibody
    • Cyp2d4 antibody
    • Cyp2d4v1 antibody
    • CYPIID18 antibody
    • Cytochrome P450 2d18 antibody
    • Cytochrome P450 subfamily IID4 antibody
    see all

References

ab22592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I understand your situation. Antibodies are expensive and committing funds to an experiment that may or may not work is a difficult decision. However, we do not have free samples or discounts for the application you want to test, immuno-inhibition. Regarding the amount of antibody that is needed to produce detectable inhibition, this will vary from one antibody to another, depending on their affinity for the protein, and the locations of the epitopes that the antibodies bind to. We do have one P450 antibody that has been tested in an inhibition assay, but it may be irrelevant to your studies. The antibody is ab22708, anti-Cytochrome P450 1A2: Click here (or use the following: https://www.abcam.com/index.html?datasheet=22708). Cross-reactivity with 2D4 is unknown, but it does not react with with recombinant human cytochromes P450 2E1, 3A4, or 2C10.

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Question
Answer

Thank you for your description of your experiment and results. This is the first report we have received of a neutralization assay with these antibodies; they have only been tested in detection assays such as IHC and western blotting or, in the case of the rabbit IgG ab27478, as a negative control for ChIP. We do not have free samples. I do not know why the addition of either antibody to the microsomes would lead to an increase in serotonin signal. I think your questions are good, and to answer them will require more controls. The incubation of antibody with serotonin will help clarify if the antibody has an effect on the signal, apart from any contribution by CYP2D4 or 5MT, and I think incubation of the antibodies with the microsomes without substrate might also be a useful control. If you have any other rabbit IgG raised against an irrelevant antigen (for example beta actin), that might also serve as an additional control, to see if the observation is general for rabbit IgG, or IgG of other species if no rabbit IgG is available. I am not sure how you are processing you samples for HPLC but I wonder if you are precipitating the substrate or serotonin in complex with the enzyme. That explanation may be unlikely, and would not seem to apply at all to the control IgG result. Regarding the optimal time for incubation of the antibodies with the enzyme or microsomes containing the enzyme, that will need to be determined by experiment, or by consulting the literature. In general, we expect most antibodies will bind to their targets within minutes, if not seconds, depending on the reaction volume and concentration of antibody and antigen.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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